FGFR2 Protein (Dimer, glycosylated, Soluble) (Fc Tag)

Catalog No. ABIN1589528

This Recombinant FGFR2 protein is produced in Insect Cells.

Quick Overview for FGFR2 Protein (Dimer, glycosylated, Soluble) (Fc Tag) (ABIN1589528)

Target: FGFR2 (Fibroblast Growth Factor Receptor 2 (FGFR2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Insect Cells

Purity: > 90 % by SDS-PAGE

Product Details

Protein Characteristics: Dimer, glycosylated, Soluble

Purification tag / Conjugate: This FGFR2 protein is labelled with Fc Tag.

Purpose: FGFR-2(IIIc)/Fc Chimera, soluble

Sequence: RPSFSLVEDT TLEPEEPPTK YQISQPEVYV AAPGESLEVR CLLKDAAVIS WTKDGVHLGP NNRTVLIGEY LQIKGATPRD SGLYACTASR TVDSETWYFM VNVTDAISSG DDEDDTDGAE DFVSENSNNK RAPYWTNTEK MEKRLHAVPA ANTVKFRCPA GGNPMPTMRW LKNGKEFKQE HRIGGYKVRN QHWSLIMESV VPSDKGNYTC VVENEYGSIN HTYHLDVVER SPHRPILQAG LPANASTVVG GDVEFVCKVY SDAQPHIQWI KHVEKNGSKY GPDGLPYLKV LKAAGVNTTD KEIEVLYIRN VTFEDAGEYT CLAGNSIGIS FHSAWLTVLP APGREKEITA SPDYLEDPRR ASIEGRGDPE EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK

Specificity: Chromosomal location:10q26

Characteristics: Length (aa):602

Application Details

Application Notes: Determined by its ability to inhibit human FGF basic-dependent proliferation on HUVE cells. The ED50 for this effect is typically at 15 - 30 ng/mL.

Comment:

Soluble Receptors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The lyophilized sFGFR-2/Fc is soluble in water and most aqueous buffers and should be reconstituted in PBS or medium to a concentration not lower than 50 μg/mL.

Buffer: PBS

Storage: -20 °C,-80 °C

Storage Comment: Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted sFGFR-2/Fc should be stored in working aliquots at -20°C.

Expiry Date: 6 months

Target Details

Target: FGFR2 (Fibroblast Growth Factor Receptor 2 (FGFR2))

Alternative Name: FGFR-2

Background: Recombinant human soluble FGFR-2 alpha (IIIc) was fused via a Xa cleavage site with the Fc part of human IgG1. Human recombinant soluble FGFR-2 alpha (IIIc) is a disulfide-linked heterodimeric protein. In the reduced form the glycosylated subunits of sFGFR-2 alpha/human Fc chimera display a molecular mass of 80-85 kDa. Fibroblast Growth Factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorigenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGFR-1 to -4 are known. Multiple forms of FGFR-1 to -3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGFR-1 and -2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the ß isoform. Only the alpha isoform has been identified for FGFR-3 and FGFR-4. Additional splicing events for FGFR-1 to -3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N-terminal half of the IgIII domain plus some intron sequences has also been reported for FGFR-1. Mutations in FGFR-1 to -3 have been found in patients with birth defects involving craniosynostosis.
Synonyms: FGFR2, BEK, JWS, CEK3, CFD1, ECT1, KGFR, TK14, TK25, BFR-1, CD332, K-SAM

Molecular Weight: 67.1 kDa

Gene ID: 2263

NCBI Accession: NM_000141, NP_000132

UniProt: P21802

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Growth Factor Binding