Endoglin Protein (ENG) (Homodimer, Soluble) (His tag)

Catalog No. ABIN1589745

Product Details

Target: Endoglin (ENG)

Protein Type: Recombinant

Protein Characteristics: Homodimer, Soluble

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This Endoglin protein is labelled with His tag.

Sequence: ERVGCDLQPV DPTRGEVTFT TSQVSEGCVA QAANAVREVH VLFLDFPGML SHLELTLQAS KQNGTETQEV FLVLVSNKNV FVKFQAPEIP LHLAYDSSLV IFQGQPRVNI TVLPSLTSRK QILDWAATKG AITSIAALDD PQSIVLQLGQ DPKAPFLCLP EAHKDMGATL EWQPRAQTPV QSCRLEGVSG HKEAYILRIL PGSEAGPRTV TVMMELSCTS GDAILILHGP PYVSWFIDIN HSMQILTTGE YSVKIFPGSK DKGVELPDTP QGLIAEARKL NASIVTSFVE LPLVSNVSLR ASSCGGVFQT TPAPVVTTPP KDTCSPVLLM SLIQPKCGNQ VMTLALNKKH VQTLQCTITG LTFWDSSCQA EDTDDHLVLS SAYSSCGMKV TAHVVSNEVI ISFPSGSPPL RKKVQCIDMD SLSFQLGLYL SPHFLQASNT IELGQQAFVQ VSVSPLTSEV TVQLDSCHLD LGPEGDMVEL IQSRTAKGSC VTLLSPSPEG DPRFSFLLRV YMVPTPTAGT LSCNLALRPS TLSQEVYKTV SMRLNIVSPD LSHHHHHH

Characteristics: Length (AA): 558
Chromosomal location: 2 B, 2 21.4 cM

Purity: > 90 % by SDS-PAGE. Visualized by silver stain

Application Details

Application Notes: Not tested so far!

Comment:

Soluble Receptors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The lyophilized sCD105 is soluble in water and most aqueous buffers and should be reconstituted in PBS or medium to a concentration not lower than 50 μg/mL.

Buffer: PBS

Storage: -20 °C/-80 °C

Storage Comment: Lyophilized samples are stable for greater than six months at -20 °C to -70 °C. Reconstituted sCD105 should be stored in working aliquots at -20 °C.

Expiry Date: 6 months

Target Details

Target: Endoglin (ENG)

Alternative Name: CD105/Endoglin (ENG Products)

Synonyms: ENG Protein, MGC137842 Protein, DKFZp469D0419 Protein, END Protein, HHT1 Protein, ORW1 Protein, AI528660 Protein, AI662476 Protein, CD105 Protein, S-endoglin Protein, endoglin Protein, ENG Protein, Eng Protein

Background: A DNA sequence encoding the extracellular domain of mouse Endoglin (Met 1 - Gly 581) was expressed in insect cells. Mouse Endoglin is a disulfide-linked homodimeric protein. Based on N-terminal sequence analysis, the primary structure of recombinant mature Endoglin starts at Glu 26. Endoglin has a calculated monomeric molecular mass of 61 kDa but as a result of glycosylation, migrates at approximately 75 - 85 kDa under reducing conditions in SDS-PAGE. Endoglin, also known as CD105, is a Type I integral membrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Two splice variants of human endoglin, the S-endoglin and L-endoglin that differ in the length of their cytoplasmic tails have been identified. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, bone marrow pro-erythroblasts, and leukemic cells of lymphoid and myeloid lineages. Human and mouse endoglin share approximately 70 % and 97 % amino acid sequence identity in their extracellular and intracellular domains, respectively. In common with betaglycan (also named TßRIII), a proteoglycan that shares regions of sequence similarity, endoglin is an accessory receptor for the TGF- superfamily ligands. Endoglin does not bind ligands by itself, but does so by associating with a ligand-binding serine/threonine kinase receptor. Endoglin binds TGF-1 and TGF-3 but not TGF-2 efficiently by associating with TGF- type II receptor (TßRII). It interacts with activin-A and BMP-7 using either the activin type II or type IIB receptors. In the case of BMP-2 which binds directly to the type I but not the type II BMP receptor, endoglin binds via either BMPR-IA (ALK-3) or BMPR-1B (ALK-6). Although the consequence of endoglin interactions on the functions of TGF- family ligands is poorly understood, endoglin has clearly been shown to be required for angiogenesis and to play a key role in heart development. Mutations in human endoglin or ALK-1 (another type I serine/threonine receptor) lead to the vascular disorder hereditary hemorrhagic telangiectasia (HHT). Mice heterozygous for endoglin have been developed as disease models for HHT. Endoglin has been shown to be a powerful marker of neovascularization. It is also useful as a functional marker that defines long-term repopulating hematopoietic stem cells.
Synonyms: Eng, CD105, AI528660, AI662476, S-endoglin

Molecular Weight: 70-75 kDa

NCBI Accession: NP_031958, NM_007932

UniProt: Q63961