N-Glycanase 1 Protein (NGLY1) (AA 2-651) (His tag)

Catalog No. ABIN1635144

Product Details

Target: N-Glycanase 1 (NGLY1)

Protein Type: Recombinant

Protein Characteristics: AA 2-651

Origin: Rat

Source: Yeast

Purification tag / Conjugate: This N-Glycanase 1 protein is labelled with His tag.

Application: ELISA

Sequence: ASATLGSSS SSASPAVAEL CQNTPETFLE ASKLLLTYAD NILRNPSDEK YRSIRIGNTA FSTRLLPVRG AVECLFEMGF EEGETHLIFP KKASVEQLQK IRDLIAVERR SRLDGSSQKV EFSQHPAAVR LPAEQPEDPT GLMQHSGNQP GQPLSLPSAP LVVGDSTIFK VLQSNIQHVQ LYENPVLQEK ALACIPVNEL KRKSQEKLFR ARKLDKGTKV SDEDFLLLEL LHWFKEEFFH WVNNVVCSRC GRETRSRDEA LPPNDDELKW GAKNVEDHYC DACQLSNRFP RYNNPEKLLE TRCGRCGEWA NCFTLCCRAL GFEARYVWDY TDHVWTEVYS PSQQRWLHCD ACEDVCDKPL LYEIGWGKKL SYIIAFSKDE VVDVTWRYSC KHEEVMSRRT KVKEELLRET INGLNKQRQL LLSESRRKEL LQRIIVELVE FISPKTPRPG ELGGRVSGSL AWRVARGETC LERKEILFIP SENEKISKQF HLRYDIVRDR YIRVSDNNAN ISGWENGVWK MESIFRKVEK DWNMVYLARK EGSSFAYISW KFECGSAGLK VDNVSIRTSS QSFETGSVRW KLRSEMAQVN LLGDRNLRSY DDFCGATEVT LEAELSRGDG DVAWQHTQLF RQSLNDHAEN GLEIIITFSD L

Specificity: Rattus norvegicus (Rat)

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: > 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.

Target Details

Target: N-Glycanase 1 (NGLY1)

Alternative Name: Peptide-N (4)- (N-acetyl-beta-glucosaminyl)asparagine amidase (Ngly1) (NGLY1 Products)

Synonyms: CDG1V Protein, PNG1 Protein, PNGase Protein, 1110002C09Rik Protein, Png1 Protein, im:6895151 Protein, zgc:110561 Protein, AtPNG1 Protein, K6M13.12 Protein, K6M13_12 Protein, peptide-N-glycanase 1 Protein, N-glycanase 1 Protein, N-glycanase 1 S homeolog Protein, peptide-N-glycanase 1 Protein, Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase Protein, similar to S. cerevisiae PNG1 (YPL096W) peptide:N-glycanase de-N-glycosylation enzyme Protein, NGLY1 Protein, ngly1.S Protein, ngly1 Protein, Ngly1 Protein, PNG1 Protein, png-1 Protein

Background: Recommended name: Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase.
Short name= PNGase.
EC= 3.5.1.52.
Alternative name(s): N-glycanase 1 Peptide:N-glycanase

UniProt: Q5XI55

Pathways: Cell RedoxHomeostasis, SARS-CoV-2 Protein Interactome