POLR2F Protein (AA 1-142) (His tag)

Catalog No. ABIN1662882

Product Details

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Protein Type: Recombinant

Protein Characteristics: AA 1-142

Origin: Schizosaccharomyces pombe

Source: Yeast

Purification tag / Conjugate: This POLR2F protein is labelled with His tag.

Application: ELISA

Sequence: MSDYEEDEAF GMDGAVMEEE VDELEMIDEN GQSQQGVSHP GEPSTTVITE DVASSKTAQS GKAVAKEDRT TTPYMTKYER ARILGTRALQ ISMNAPVLVD LEGETDPLQI AMKELAQKKI PLLVRRYLPD GSYEDWSVAE LI

Specificity: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: > 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.

Target Details

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Alternative Name: DNA-directed RNA polymerases I, II, and III subunit RPABC2 (rpb6) (POLR2F Products)

Synonyms: HRBP14.4 Protein, POLRF Protein, RPABC2 Protein, RPB14.4 Protein, RPB6 Protein, 1810060D16Rik Protein, RNA polymerase II subunit F Protein, polymerase (RNA) II (DNA directed) polypeptide F Protein, POLR2F Protein, Polr2f Protein

Background: Recommended name: DNA-directed RNA polymerases I, II, and III subunit RPABC2.
Short name= RNA polymerases I, II, and III subunit ABC2.
Alternative name(s): DNA-directed RNA polymerases I, II, and III 15 kDa polypeptide RPC16

UniProt: P36595

Pathways: Regulatory RNA Pathways