Chemokine (C-C Motif) Ligand 12 (Ccl12) (Active) Protein

Details for Product No. ABIN1686358
Protein Name
Origin
Mouse (Murine)
17
Source
Escherichia coli (E. coli)
14
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
High Pressure Liquid Chromatography (HPLC), SDS-PAGE (SDS)
Options
Sequence GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG
Purity ≥98 % by SDS-PAGE
Sterility 0.2 μm filtered
Endotoxin Level < 0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 10.0-50.0 ng/ml.

Specification / Test Method: Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 10.0-50.0 ng/ml.
Protein Name
Background The MCP proteins belong to the CC chemokine family, and signal through CCR2 and, with the exception of MCP-1, other CCR receptors. The MCP proteins chemoattract and activate monocytes, activated T cells, basophils, NK cells, and immature dendritic cells. The MCP family cross- reacts across species. Recombinant murine MCP-5 is a 9.3 kDa protein containing 82 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

Alternative Names: Monocyte Chemotactic Protein-5, CCL12
Molecular Weight 9.3 kDa
Gene ID 20293
UniProt Q62401
Pathways Cellular Response to Molecule of Bacterial Origin
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8 °C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1 % BSA) and store in working aliquots at -20 °C to -80 °C.
Buffer Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.
Preservative Without preservative
Handling Advice Centrifuge the vial prior to opening.
Storage -20 °C
Expiry Date 12 months