Elastase 3A Protein (ELA3A) (AA 16-270) (His tag)
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- Target See all Elastase 3A (ELA3A) products
- Elastase 3A (ELA3A)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 16-270
- Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This Elastase 3A protein is labelled with His tag.
- Purpose
- Recombinant Human Chymotrypsin-Like Elastase Family Member 3A/CELA3A (C-6His)
- Sequence
- SGYGPPSSHS SSRVVHGEDA VPYSWPWQVS LQYEKSGSFY HTCGGSLIAP DWVVTAGHCI SRDLTYQVVL GEYNLAVKEG PEQVIPINSE ELFVHPLWNR SCVACGNDIA LIKLSRSAQL GDAVQLASLP PAGDILPNKT PCYITGWGRL YTNGPLPDKL QQARLPVVDY KHCSRWNWWG STVKKTMVCA GGYIRSGCNG DSGGPLNCPT EDGGWQVHGV TSFVSGFGCN FIWKPTVFTR VSAFIDWIEE TIASHVDHHH HHH
- Characteristics
- Recombinant Human Chymotrypsin-Like Elastase Family Member 3A/CELA3A is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Ser16-His270) of Human CELA3A fused with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Elastase 3A (ELA3A) (AA 16-270) protein (GST tag)
ELA3A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Elastase 3A (ELA3A) (AA 29-270) protein (His tag)Crystallography grade ELA3A Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Elastase 3A (ELA3A) (AA 29-270) protein (His-SUMO Tag)ELA3A Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,10 % Glycerol, pH 7.5.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- Elastase 3A (ELA3A)
- Alternative Name
- CELA3A (ELA3A Products)
- Synonyms
- ELA3 Protein, ELA3A Protein, ela3a Protein, chymotrypsin like elastase family member 3A Protein, chymotrypsin like elastase family member 3A S homeolog Protein, CELA3A Protein, cela3a.S Protein, cela3a Protein
- Sub Type
- Fusionprotein
- Background
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Chymotrypsin-Like Elastase Family Member 3A (CELA3A) is an enzyme that contains one peptidase S1 domain. ELA3A belongs to the peptidase S1 family of the Elastase subfamily. ELA3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. ELA3A may also function in the intestinal transport and metabolism of cholesterol. ELA3A is efficient protease with alanine specificity but only little elastolytic activity. ELA3A preferentially cleaves proteins after alanine residues.
Alternative Names: Chymotrypsin-Like Elastase Family Member 3A, Elastase IIIA, Elastase-3A, Protease E, CELA3A, ELA3, ELA3A - Molecular Weight
- 28.9 kDa
- UniProt
- P09093
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