SMPDL3A Protein (AA 23-453) (His tag)

Catalog No. ABIN1691722

Product Details

Target: SMPDL3A (Sphingomyelin phosphodiesterase, Acid-Like 3A (SMPDL3A))

Protein Type: Recombinant

Protein Characteristics: AA 23-453

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This SMPDL3A protein is labelled with His tag.

Purpose: Recombinant Human ASM-Like Phosphodiesterase 3a/SMPDL3A (C-6His)

Sequence: LPVAPAGGRN PPPAIGQFWH VTDLHLDPTY HITDDHTKVC ASSKGANASN PGPFGDVLCD SPYQLILSAF DFIKNSGQEA SFMIWTGDSP PHVPVPELST DTVINVITNM TTTIQSLFPN LQVFPALGNH DYWPQDQLPV VTSKVYNAVA NLWKPWLDEE AISTLRKGGF YSQKVTTNPN LRIISLNTNL YYGPNIMTLN KTDPANQFEW LESTLNNSQQ NKEKVYIIAH VPVGYLPSSQ NITAMREYYN EKLIDIFQKY SDVIAGQFYG HTHRDSIMVL SDKKGSPVNS LFVAPAVTPV KSVLEKQTNN PGIRLFQYDP RDYKLLDMLQ YYLNLTEANL KGESIWKLEY ILTQTYDIED LQPESLYGLA KQFTILDSKQ FIKYYNYFFV SYDSSVTCDK TCKAFQICAI MNLDNISYAD CLKQLYIKHN YVDHHHHHH

Characteristics: Recombinant Human Acid sphingomyelinase-like phosphodiesterase 3a/SMPDL3A is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Leu23-Tyr453) of Human SMPDL3A fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 5 months

Target Details

Target: SMPDL3A (Sphingomyelin phosphodiesterase, Acid-Like 3A (SMPDL3A))

Alternative Name: SMPDL3A (SMPDL3A Products)

Synonyms: ASM3A Protein, ASML3a Protein, yR36GH4.1 Protein, 0610010C24Rik Protein, AI529588 Protein, ASML3 Protein, ASML3A Protein, sphingomyelin phosphodiesterase acid like 3A Protein, sphingomyelin phosphodiesterase, acid-like 3A Protein, SMPDL3A Protein, Smpdl3a Protein

Sub Type: Fusionprotein

Background: Acid sphingomyelinase-like phosphodiesterase 3a (SMPDL3A ) is a novel liver X receptor (LXR) -regulated gene, with an LXR response element within its promoter. The induction of SMPDL3A is LXR-dependent and is restricted to human blood cells with no induction observed in mouse cellular systems. LXR and LXR function as physiological sensors of cholesterol metabolites (oxysterols), regulating key genes involved in cholesterol and lipid metabolism. LXRs have been extensively studied in both human and rodent cell systems, revealing their potential therapeutic value in the contexts of atherosclerosis and inflammatory diseases. The LXR genome landscape has been investigated in murine macrophages but not in human THP-1 cells, which represent one of the frequently used monocyte/macrophage cell systems to study immune responses.
Alternative Names:

Molecular Weight: 49.9 kDa

UniProt: Q92484