LYPLA2 Protein (AA 1-231) (His tag)
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- Target See all LYPLA2 Proteins
- LYPLA2 (Lysophospholipase II (LYPLA2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-231
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This LYPLA2 protein is labelled with His tag.
- Purpose
- Recombinant Human Acyl-Protein Thioesterase 2/LYPLA2/APT2 (C-6His)
- Sequence
- MCGNTMSVPL LTDAATVSGA ERETAAVIFL HGLGDTGHSW ADALSTIRLP HVKYICPHAP RIPVTLNMKM VMPSWFDLMG LSPDAPEDEA GIKKAAENIK ALIEHEMKNG IPANRIVLGG FSQGGALSLY TALTCPHPLA GIVALSCWLP LHRAFPQAAN GSAKDLAILQ CHGELDPMVP VRFGALTAEK LRSVVTPARV QFKTYPGVMH SSCPQEMAAV KEFLEKLLPP VLEHHHHHH
- Characteristics
- Recombinant Human Acyl-Protein Thioesterase 2/LYPLA2 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Val231) of Human LYPLA2 fused with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product LYPLA2 Protein
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Lysophospholipase II (LYPLA2) (AA 144-231) protein (GST tag)
LYPLA2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Lysophospholipase II (LYPLA2) (AA 1-231) protein (GST tag)LYPLA2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Lysophospholipase II (LYPLA2) (AA 1-231) protein (Strep Tag)Crystallography grade LYPLA2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Lysophospholipase II (LYPLA2) (AA 1-231) protein (Strep Tag)Crystallography grade LYPLA2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Lysophospholipase II (LYPLA2) protein (Myc-DYKDDDDK Tag)LYPLA2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM Tris, 1 mM DTT, 0.1M NaCl, pH 8.0.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- LYPLA2 (Lysophospholipase II (LYPLA2))
- Alternative Name
- Acyl-Protein Thioesterase 2/LYPLA2 (LYPLA2 Products)
- Synonyms
- APT-2 Protein, DJ886K2.4 Protein, LysoII Protein, MGC52664 Protein, MGC75683 Protein, LYPLA2P1 Protein, lysophospholipase II Protein, lysophospholipase 2 Protein, lysophospholipase II S homeolog Protein, LYPLA2 Protein, Lypla2 Protein, lypla2.S Protein, lypla2 Protein
- Sub Type
- Fusionprotein
- Background
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Acyl-Protein Thioesterase 2 is a cytoplasmic protein that belongs to the AB Hydrolase 2 family. LYPLA2 is a lysophospholipase and hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. LYPLA2 has hydrolase activity that converts Palmitoyl-protein to palmitate and protein. LYPLA2 regulates the multifunctional lysophospholipids by acting on biological membranes. LYPLA2 participates in Glycerophospholipid metabolism pathway, fatty acid metabolic process and lipid metabolic process.
Alternative Names: Acyl-Protein Thioesterase 2, APT-2, Lysophospholipase II, LPL-II, LysoPLA II, LYPLA2, APT2 - Molecular Weight
- 25.8 kDa
- UniProt
- O95372
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