ALDH1A1 Protein (AA 2-501) (His tag)

Catalog No. ABIN1692069

Product Details

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Protein Type: Recombinant

Protein Characteristics: AA 2-501

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ALDH1A1 protein is labelled with His tag.

Purpose: Recombinant Human Aldehyde Dehydrogenase 1-A1/ALDH1A1 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MSSSGTPDLP VLLTDLKIQY TKIFINNEWH DSVSGKKFPV FNPATEEELC QVEEGDKEDV DKAVKAARQA FQIGSPWRTM DASERGRLLY KLADLIERDR LLLATMESMN GGKLYSNAYL NDLAGCIKTL RYCAGWADKI QGRTIPIDGN FFTYTRHEPI GVCGQIIPWN FPLVMLIWKI GPALSCGNTV VVKPAEQTPL TALHVASLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDID KVAFTGSTEV GKLIKEAAGK SNLKRVTLEL GGKSPCIVLA DADLDNAVEF AHHGVFYHQG QCCIAASRIF VEESIYDEFV RRSVERAKKY ILGNPLTPGV TQGPQIDKEQ YDKILDLIES GKKEGAKLEC GGGPWGNKGY FVQPTVFSNV TDEMRIAKEE IFGPVQQIMK FKSLDDVIKR ANNTFYGLSA GVFTKDIDKA ITISSALQAG TVWVNCYGVV SAQCPFGGFK MSGNGRELGE YGFHEYTEVK TVTVKISQKN S

Characteristics: Recombinant Human Aldehyde Dehydrogenase Family 1 Member A1/ALDH1A1 is produced with our E. coli expression system. The target protein is expressed with sequence (Ser2-Ser501) of Human ALDH1A1 fused with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, pH 8.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Alternative Name: ALDH1A1 (ALDH1A1 Products)

Synonyms: ALDC Protein, ALDH-E1 Protein, ALDH1 Protein, ALDH11 Protein, PUMB1 Protein, RALDH1 Protein, AL1A1 Protein, aldc Protein, aldh-e1 Protein, aldh1 Protein, aldh11 Protein, pumb1 Protein, raldh1 Protein, GGADHR Protein, ALDH1A1 Protein, ALDDH Protein, Ahd2 Protein, Aldh1 Protein, Aldh2 Protein, Ahd-2 Protein, Aldh1a2 Protein, E1 Protein, Raldh1 Protein, Aldh1a1 Protein, ALHDII Protein, RALDH 1 Protein, RalDH1 Protein, aldehyde dehydrogenase 1 family member A1 Protein, aldehyde dehydrogenase 1 family, member A1 Protein, aldehyde dehydrogenase 1 family member A1 L homeolog Protein, aldehyde dehydrogenase family 1, subfamily A1 Protein, aldehyde dehydrogenase Protein, retinal dehydrogenase 1 Protein, ALDH1A1 Protein, aldh1a1 Protein, Aldh1a1 Protein, aldh1a1.L Protein, aldH1 Protein, LOC100732581 Protein, LOC101822890 Protein

Sub Type: Fusionprotein

Background: Aldehyde Dehydrogenase Family 1 Member A1 (ALDH1A1) is a cytoplasmic enzyme that belongs to the Aldehyde Dehydrogenase family. ALDH1A1 is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties and subcellular localizations. ALDH1A1 is the main cytosolic isoform, which has a lower affinity for aldehydes than the mitochondrial enzyme. ALDH1A1 binds free retinal and cellular retinol-binding protein-bound retinal. It can convert/oxidize retinaldehyde to retinoic acid.
Alternative Names: Retinal Dehydrogenase 1, RALDH 1, RalDH1, ALDH-E1, ALHDII, Aldehyde Dehydrogenase Family 1 Member A1, Aldehyde Dehydrogenase Cytosolic, ALDH1A1, ALDC, ALDH1, PUMB1

Molecular Weight: 57 kDa

UniProt: P00352

Pathways: Dopaminergic Neurogenesis