NCK1 Protein (AA 1-377) (His tag)

Catalog No. ABIN1692085

Product Details

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Protein Type: Recombinant

Protein Characteristics: AA 1-377

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This NCK1 protein is labelled with His tag.

Purpose: Recombinant Human Cytoplasmic Protein NCK1 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MAEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKSWWR VRNSMNKTGF VPSNYVERKN SARKASIVKN LKDTLGIGKV KRKPSVPDSA SPADDSFVDP GERLYDLNMP AYVKFNYMAE REDELSLIKG TKVIVMEKCS DGWWRGSYNG QVGWFPSNYV TEEGDSPLGD HVGSLSEKLA AVVNNLNTGQ VLHVVQALYP FSSSNDEELN FEKGDVMDVI EKPENDPEWW KCRKINGMVG LVPKNYVTVM QNNPLTSGLE PSPPQCDYIR PSLTGKFAGN PWYYGKVTRH QAEMALNERG HEGDFLIRDS ESSPNDFSVS LKAQGKNKHF KVQLKETVYC IGQRKFSTME ELVEHYKKAP IFTSEQGEKL YLVKHLS

Characteristics: Recombinant Human Cytoplasmic Protein NCK1/NCK1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Ser377) of Human NCK1 fused with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 50 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Alternative Name: NCK1 (NCK1 Products)

Synonyms: NCK Protein, NCKalpha Protein, nck-1 Protein, NCK1 Protein, 6330586M15Rik Protein, D230010O13Rik Protein, Nck Protein, Nck-1 Protein, Nck1 Protein, nck Protein, nck1-a Protein, nck1-b Protein, nckalpha Protein, NCK adaptor protein 1 Protein, non-catalytic region of tyrosine kinase adaptor protein 1 Protein, NCK adaptor protein 1b Protein, NCK adaptor protein 1 L homeolog Protein, NCK1 Protein, Nck1 Protein, nck1b Protein, nck1.L Protein

Sub Type: Fusionprotein

Background: Cytoplasmic Protein NCK1 (NCK1) is a cytoplasmic protein that contains one SH2 domain and three SH3 domains. NCK1 is a member of the adapter family, which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. NCK1 maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. NCK1 plays a role in the DNA damage response, but not in the detection of the damage by ATM/ATR. It is also involved in transducing signals from receptor tyrosine kinases to downstream signal recipients, such as ELK1-dependent transcriptional activation in response to activated Ras signaling.
Alternative Names: Cytoplasmic Protein NCK1, NCK adaptor Protein 1, Nck-1, SH2/SH3 Adaptor Protein NCK-Alpha, NCK1, NCK

Molecular Weight: 45 kDa

UniProt: P16333

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals