BLK Protein (AA 2-505) (His tag)

Catalog No. ABIN1692105

Product Details

Target: BLK (B Lymphoid Tyrosine Kinase (BLK))

Protein Type: Recombinant

Protein Characteristics: AA 2-505

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This BLK protein is labelled with His tag.

Purpose: Recombinant Human Tyrosine-Protein Kinase Blk/BLK (C-6His)

Sequence: GLVSSKKPDK EKPIKEKDKG QWSPLKVSAQ DKDAPPLPPL VVFNHLTPPP PDEHLDEDKH FVVALYDYTA MNDRDLQMLK GEKLQVLKGT GDWWLARSLV TGREGYVPSN FVARVESLEM ERWFFRSQGR KEAERQLLAP INKAGSFLIR ESETNKGAFS LSVKDVTTQG ELIKHYKIRC LDEGGYYISP RITFPSLQAL VQHYSKKGDG LCQRLTLPCV RPAPQNPWAQ DEWEIPRQSL RLVRKLGSGQ FGEVWMGYYK NNMKVAIKTL KEGTMSPEAF LGEANVMKAL QHERLVRLYA VVTKEPIYIV TEYMARGCLL DFLKTDEGSR LSLPRLIDMS AQIAEGMAYI ERMNSIHRDL RAANILVSEA LCCKIADFGL ARIIDSEYTA QEGAKFPIKW TAPEAIHFGV FTIKADVWSF GVLLMEVVTY GRVPYPGMSN PEVIRNLERG YRMPRPDTCP PELYRGVIAE CWRSRPEERP TFEFLQSVLE DFYTATERQY ELQPLEHHHH HH

Characteristics: Recombinant Human Tyrosine-Protein Kinase Blk/BLK is produced with our E. coli expression system. The target protein is expressed with sequence (Gly2-Pro505) of Human BLK fused with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 1 mM DTT, pH 7.4.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: BLK (B Lymphoid Tyrosine Kinase (BLK))

Alternative Name: Tyrosine-Protein Kinase Blk/BLK (BLK Products)

Synonyms: bltk Protein, si:dkey-33i22.2 Protein, zgc:136231 Protein, MODY11 Protein, BLK proto-oncogene, Src family tyrosine kinase Protein, B lymphoid kinase Protein, blk Protein, BLK Protein, Blk Protein

Sub Type: Fusionprotein

Background: Tyrosine-Protein Kinase Blk (BLK) contains one protein kinase domain, one SH2 domain and one SH3 domain. BLK is a non-receptor tyrosine kinase, which is involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulines and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Defects in BLK are a cause of maturity-onset diabetes of the young type 11 (MODY11).
Alternative Names: Tyrosine-Protein Kinase Blk, B Lymphocyte Kinase, p55-Blk, BLK

Molecular Weight: 58.7 kDa

UniProt: P51451

Pathways: Positive Regulation of Peptide Hormone Secretion, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling