NANS Protein (AA 1-359) (His tag)
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- Target See all NANS Proteins
- NANS (N-Acetylneuraminic Acid Synthase (NANS))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-359
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NANS protein is labelled with His tag.
- Purpose
- Recombinant Human N-Acetylneuraminate Synthase/NANS/SAS (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MPLELELCPG RWVGGQHPCF IIAEIGQNHQ GDLDVAKRMI RMAKECGADC AKFQKSELEF KFNRKALDRP YTSKHSWGKT YGEHKRHLEF SHDQYRELQR YAEEVGIFFT ASGMDEMAVE FLHELNVPFF KVGSGDTNNF PYLEKTAKKG RPMVISSGMQ SMDTMKQVYQ IVKPLNPNFC FLQCTSAYPL QPEDVNLRVI SEYQKLFPDI PIGYSGHETG IAISVAAVAL GAKVLERHIT LDKTWKGSDH SASLEPGELA ELVRSVRLVE RALGSPTKQL LPCEMACNEK LGKSVVAKVK IPEGTILTMD MLTVKVGEPK GYPPEDIFNL VGKKVLVTVE EDDTIMEELV DNHGKKIKS
- Characteristics
- Recombinant Human N-Acetylneuraminate Synthase/NANS is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Ser359) of Human NANS fused with a His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product NANS Protein
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N-Acetylneuraminic Acid Synthase (NANS) (AA 1-359) protein (His tag)
NANS Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
N-Acetylneuraminic Acid Synthase (NANS) (AA 1-359) protein (GST tag)NANS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
N-Acetylneuraminic Acid Synthase (NANS) (AA 260-359) protein (GST tag)NANS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
N-Acetylneuraminic Acid Synthase (NANS) (AA 2-359) protein (His tag)Crystallography grade NANS Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
N-Acetylneuraminic Acid Synthase (NANS) protein (Myc-DYKDDDDK Tag)NANS Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM Tris, 100 mM NaCl, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- NANS (N-Acetylneuraminic Acid Synthase (NANS))
- Alternative Name
- NANS (NANS Products)
- Synonyms
- MGC84329 Protein, SAS Protein, N-acetylneuraminate synthase L homeolog Protein, N-acetylneuraminate synthase Protein, nans.L Protein, NANS Protein, Nans Protein
- Sub Type
- Fusionprotein
- Background
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Sialic Acid Synthase (NANS) is an enzyme that contains one AFP-like domain. NANS is ubiquitous and plays a role in the biosynthetic pathways of sialic acids. NANS produces N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN). It also can use N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively.
Alternative Names: Sialic Acid Synthase, N-Acetylneuraminate Synthase, N-Acetylneuraminate-9-Phosphate Synthase, N-Acetylneuraminic Acid Phosphate Synthase, N-Acetylneuraminic Acid Synthase, NANS, SAS - Molecular Weight
- 42.4 kDa
- UniProt
- Q9NR45
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