CLIC1 Protein (AA 1-241) (His tag)

Catalog No. ABIN1692285

Product Details

Target: CLIC1 (Chloride Intracellular Channel 1 (CLIC1))

Protein Type: Recombinant

Protein Characteristics: AA 1-241

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CLIC1 protein is labelled with His tag.

Purpose: Recombinant Human Chloride Intracellular Channel Protein 1/CLIC1 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MAEEQPQVEL FVKAGSDGAK IGNCPFSQRL FMVLWLKGVT FNVTTVDTKR RTETVQKLCP GGQLPFLLYG TEVHTDTNKI EEFLEAVLCP PRYPKLAALN PESNTAGLDI FAKFSAYIKN SNPALNDNLE KGLLKALKVL DNYLTSPLPE EVDETSAEDE GVSQRKFLDG NELTLADCNL LPKLHIVQVV CKKYRGFTIP EAFRGVHRYL SNAYAREEFA STCPDDEEIE LAYEQVAKAL K

Characteristics: Recombinant Human Chloride Intracellular Channel Protein 1/CLIC1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Lys241) of Human CLIC1 fused with a His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: CLIC1 (Chloride Intracellular Channel 1 (CLIC1))

Alternative Name: CLIC1 (CLIC1 Products)

Synonyms: ncc27 Protein, xclic1 Protein, MGC75951 Protein, MGC132377 Protein, CLIC1 Protein, G6 Protein, NCC27 Protein, Clcp Protein, wu:fc30e04 Protein, zgc:77044 Protein, chloride intracellular channel 1 Protein, chloride intracellular channel 1 S homeolog Protein, clic1 Protein, CLIC1 Protein, Clic1 Protein, clic1.S Protein

Sub Type: Fusionprotein

Background: Chloride Intracellular Channel Protein 1 (CLIC1) belongs to the Chloride Channel CLIC family and contains one GST C-terminal domain. CLIC1 can be expressed in various cell types, but it is especially prominent in the heart, placenta, liver, kidney, and pancreas. CLIC1 can insert into membranes and form chloride ion channels. The channel activity depends on the pH. CLIC1 membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. CLIC1 is also involved in the regulation of the cell cycle.
Alternative Names: Chloride Intracellular Channel Protein 1, Chloride Channel ABP, Nuclear Chloride Ion Channel 27, NCC27, Regulatory Nuclear Chloride Ion Channel Protein, hRNCC, CLIC1, G6, NCC27

Molecular Weight: 29 kDa

UniProt: O00299