UBE2V1 Protein (AA 2-147) (His tag)

Catalog No. ABIN1692351

Product Details

Target: UBE2V1 (Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1))

Protein Type: Recombinant

Protein Characteristics: AA 2-147

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2V1 protein is labelled with His tag.

Purpose: Recombinant Human Ubiquitin-Conjugating Enzyme E2 Variant 1/UBE2V1 (C-6His)

Sequence: AATTGSGVKV PRNFRLLEEL EEGQKGVGDG TVSWGLEDDE DMTLTRWTGM IIGPPRTIYE NRIYSLKIEC GPKYPEAPPF VRFVTKINMN GVNSSNGVVD PRAISVLAKW QNSYSIKVVL QELRRLMMSK ENMKLPQPPE GQCYSNLEHH HHHH

Characteristics: Recombinant Human Ubiquitin-Conjugating Enzyme E2 Variant 1/UBE2V1 is produced with our E. coli expression system. The target protein is expressed with sequence (Ala2-Asn147) of Human UBE2V1 fused with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 100 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: UBE2V1 (Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1))

Alternative Name: UBE2V1 (UBE2V1 Products)

Synonyms: CIR1 Protein, CROC-1 Protein, CROC1 Protein, UBE2V Protein, UEV-1 Protein, UEV1 Protein, UEV1A Protein, 0610011J09Rik Protein, AI256840 Protein, D7Bwg1382e Protein, CROC-1B Protein, ubiquitin conjugating enzyme E2 V1 Protein, ubiquitin-conjugating enzyme E2 variant 1 Protein, UBE2V1 Protein, ube2v1 Protein, Ube2v1 Protein

Sub Type: Fusionprotein

Background: Ubiquitin-Conjugating Enzyme Variant 1a (UBE2V1) is a member of the Ubiquitin-conjugating (E2) enzyme family. The E2 catalytic core domain of UBE2V1 lacks an active site cysteine residue, rendering it catalytically inactive on its own. However, in the cytoplasm UBE2V1 is able to form a catalytically active complex with UBE2N/Ubc13, which mediates the synthesis Lys63-linked Ubiquitin chains and is required for NF-kappa B activation. UBE2V1 is required for UBE2N (Ubc13)/UBE2V1 Complex-dependent Lys63-linked Ubiquitin chain formation. More specifically, UBE2V1 orients the Ubiquitin molecule to favor linkage at Lys63 via a non-covalent interaction with the Ubiquitin molecule. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. UBE2V1 plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6, and TRAF2. It mediates transcriptional activation of target genes. UBE2V1 also controls the progress through the cell cycle and differentiation, the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.
Alternative Names: Ubiquitin-Conjugating Enzyme E2 Variant 1, UEV-1, CROC-1, TRAF6-Regulated IKK Activator 1 Beta Uev1A, UBE2V1, CROC1, UBE2V, UEV1, P/OKcl.19

Molecular Weight: 17.5 kDa

UniProt: Q13404

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades