Growth Hormone 1 Protein (GH1) (His tag)

Catalog No. ABIN1719793

Product Details

Target: Growth Hormone 1 (GH1)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Tobacco (Nicotiana benthamiana)

Purification tag / Conjugate: This Growth Hormone 1 protein is labelled with His tag.

Application: Western Blotting (WB), Cell Culture (CC)

Sequence: HHHHHHFPTI PLSRPFDNAM LRAHRLHQLA FDTYQEFEEA YIPKEQKYSF LQNPQTSLCF SESIPTPSNR EETQQKSNLE LLRISLLLIQ SWLEPVQFLR SVFANSLVYG ASDSNVYDLL KDLEEGIQTL MGRLEDGSPR TGQIFKQTYS KFDTNSHNDD ALLKNYGLLY CFRKDMDKVE TFLRIVQCRS VEGSCGFAG

Characteristics: Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody HGH. (Fig.1 Lanes 4-5).
Molecular formula: C1025H1570N280O306S7.
Isoelectric Point: 5,98.
Extinction coefficient: E 0.1 % (1g/L) = 0.77 (A 280 nm).
This product contains no animal-derived components or impurities. Produced by transient expression of human Growth Hormone in non-transgenic plants.

Purification: Recombinant human Growth Hormone contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC).

Purity: > 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Endotoxin Level: < 0.04 EU/µg protein (LAL method)

Application Details

Comment:

Biological Activity: The biological activity of human Growth Hormone is measured by cell proliferation using Nb2-11 cells. ED50 ≤ 0.04-0.1 ng/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized protein should be reconstituted in water to a concentration of 200 ng/µL.

Buffer: PBS 0.05 M buffer at pH 7.5

Storage: 4 °C

References

Bults, Sonesson, Knutsson, Bischoff, van de Merbel: "Intact protein quantification in biological samples by liquid chromatography - high-resolution mass spectrometry: somatropin in rat plasma." in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, Vol. 1144, pp. 122079, (2020) (PubMed).

Target Details

Target: Growth Hormone 1 (GH1)

Alternative Name: GH1 (GH1 Products)

Synonyms: GH Protein, GH-N Protein, GHN Protein, IGHD1B Protein, hGH-N Protein, Gh1 Protein, GH1 Protein, gh Protein, Gh Protein, GH2 Protein, RNGHGP Protein, ghl Protein, gh-n Protein, ghn Protein, ighd1b Protein, gh1 Protein, ghb-A Protein, GHI Protein, GHB3 Protein, growth hormone 1 Protein, growth hormone Protein, somatotropin-like Protein, somatotropin Protein, growth hormone prepeptide Protein, growth hormone 1 L homeolog Protein, growth hormone 1 S homeolog Protein, Somatotropin-1 Protein, GH1 Protein, Gh Protein, GH Protein, LOC100305005 Protein, Gh1 Protein, LOC100534452 Protein, LOC100232594 Protein, LOC100303681 Protein, gh1 Protein, LOC100356068 Protein, LOC100136588 Protein, gh1.L Protein, gh1.S Protein, LOC109081196 Protein

Background: Synonyms: GH, GH-N, GHN, IGHD1B, hGH-N, pituitary growth hormone, somatotropin
GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation, an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.

Molecular Weight: 22.9 kDa

Gene ID: 2688, 139250

Pathways: NF-kappaB Signaling, JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process