PARP1 Protein

Catalog No. ABIN1741730

Product Details

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus

Application: Western Blotting (WB), ELISA

Characteristics: PARP1 Automodified is highly purified human PARP1, expressed in a baculovirus expression system, to which NAD+ and cofactors have been added to allow PARP automodification by poly(ADP-ribose). The PARP1 is lyophilized from its native state, and upon reconstitution with water, it retains residual poly ADP-ribosylation enzymatic activity. It is useful as a standard for ELISA, SDS-PAGE, Western blotting, and in other assays.
Full-length hPARP1

Purification: Affinity chromatography

Purity: > 95 %

Application Details

Application Notes: 100 ng/lane WB, 10 ng/well ELISA

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconsitute in dH2O

Storage: -20 °C

References

Zhang, Wang, Ding, Yu: "Site-specific characterization of the Asp- and Glu-ADP-ribosylated proteome." in: Nature methods, Vol. 10, Issue 10, pp. 981-4, (2013) (PubMed).

Target Details

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: Poly ADP ribose Polymerase 1 (PARP1 Products)

Synonyms: ADPRT Protein, ADPRT 1 Protein, ADPRT1 Protein, ARTD1 Protein, PARP Protein, PARP-1 Protein, PPOL Protein, pADPRT-1 Protein, Adprt Protein, Parp-1 Protein, 5830444G22Rik Protein, AI893648 Protein, Adprp Protein, Adprt1 Protein, C80510 Protein, parp-1 Protein, sPARP-1 Protein, BEST:LD21673 Protein, CG17685 Protein, CG17696 Protein, CG17718 Protein, CG40411 Protein, D.PARP Protein, Dm.pARTa Protein, Dmel\\CG40411 Protein, LD21673.3prime Protein, PARP1 Protein, dPARP Protein, parp Protein, adprt1 Protein, padprt-1 Protein, ppol Protein, si:dkey-206f10.3 Protein, wu:fc60f12 Protein, zgc:110092 Protein, poly(ADP-ribose) polymerase 1 Protein, poly (ADP-ribose) polymerase 1 Protein, poly (ADP-ribose) polymerase family, member 1 Protein, Poly-(ADP-ribose) polymerase Protein, poly(ADP-ribose) polymerase 1 L homeolog Protein, PARP1 Protein, Parp1 Protein, Parp Protein, parp1.L Protein, parp1 Protein

Background: Poly (ADP-ribose) polymerase-1 (PARP1) is an abundant and ubiquitous nuclear enzyme that catalyzes the NAD(+)-dependent addition of ADP-ribose polymers on a variety of nuclear proteins.

Molecular Weight: >113 kDa

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location