CD320 Protein (AA 36-231) (His tag)
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- Target See all CD320 Proteins
- CD320 (CD320 Molecule (CD320))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 36-231
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This CD320 protein is labelled with His tag.
- Purpose
- Recombinant Human Transcobalamin II Receptor/TCblR/8D6A/CD320 (C-6His)
- Sequence
- SPLSTPTSAQ AAGPSSGSCP PTKFQCRTSG LCVPLTWRCD RDLDCSDGSD EEECRIEPCT QKGQCPPPPG LPCPCTGVSD CSGGTDKKLR NCSRLACLAG ELRCTLSDDC IPLTWRCDGH PDCPDSSDEL GCGTNEILPE GDATTMGPPV TLESVTSLRN ATTMGPPVTL ESVPSVGNAT SSSAGDQSGS PTAYGVVDHH HHHH
- Characteristics
- Recombinant Human CD320/TCblR/8D6A is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Ser36-Val231) of Human CD320 fused with a FC tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product CD320 Protein
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CD320 Molecule (CD320) (AA 97-196) protein (GST tag)
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CD320 Molecule (CD320) (AA 1-282) protein (GST tag)CD320 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
CD320 Molecule (CD320) (AA 36-229) protein (His tag)Crystallography grade CD320 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
CD320 Molecule (CD320) (AA 29-208) protein (His tag)Crystallography grade CD320 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
CD320 Molecule (CD320) (AA 36-282) protein (rho-1D4 tag)Crystallography grade CD320 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
CD320 Molecule (CD320) (AA 29-260) protein (rho-1D4 tag)Crystallography grade CD320 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-Citrate,150 mM NaCl, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- CD320 (CD320 Molecule (CD320))
- Alternative Name
- CD320 (CD320 Products)
- Background
- CD320 antigen is also known as 8D6 antigen,FDC-signaling molecule 8D6,Transcobalamin receptor and 8D6A. It is a single-pass type I membrane protein and containing two LDL-receptor class A domains. CD320 has been recently discovered and reported as a follicular dendritic cell (FDC) protein. CD320 can augments the proliferation of plasma cells precursors generated by IL-10. CD320 also founctions a receptor for the cellular uptake of transcobalamin bound cobalamin. Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC) which is a metabolic disorder.
- Molecular Weight
- 21.1 kDa
- UniProt
- Q9NPF0
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