Ephrin B1 Protein (EFNB1) (AA 28-232) (His tag)
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- Target See all Ephrin B1 (EFNB1) Proteins
- Ephrin B1 (EFNB1)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 28-232
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This Ephrin B1 protein is labelled with His tag.
- Purpose
- Recombinant Human Ephrin-B1/EFNB1 (C-6His)
- Sequence
- LAKNLEPVSW SSLNPKFLSG KGLVIYPKIG DKLDIICPRA EAGRPYEYYK LYLVRPEQAA ACSTVLDPNV LVTCNRPEQE IRFTIKFQEF SPNYMGLEFK KHHDYYITST SNGSLEGLEN REGGVCRTRT MKIIMKVGQD PNAVTPEQLT TSRPSKEADN TVKMATQAPG SRGSLGDSDG KHETVNQEEK SGPGASGGSS GDPDGVDHHH HHH
- Characteristics
- Recombinant Human Ephrin-B1/EFNB1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Leu28-Gly232) of Human EFNB1 fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product EFNB1 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- Ephrin B1 (EFNB1)
- Alternative Name
- Ephrin-B1/EFNB1 (EFNB1 Products)
- Sub Type
- Fusionprotein
- Background
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Ephrin-B1, also named EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2, is a single-pass type I membrane protein. It contains 1 ephrin RBD (ephrin receptor-binding) domain and belongs to the ephrin family. Ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. All ephrins share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. Ephrin-B1 has been shown to bind EphA3, EphB1, EphB2, EphB3, and EphB4. The extracellular domains of human and mouse ephrin-B1 share 94% amino acid identity.
Alternative Names: Ephrin-B1, also named EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2, is a single-pass type I membrane protein. - Molecular Weight
- 23.4 kDa
- UniProt
- P98172
- Pathways
- RTK Signaling
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