SPOCK3 Protein (AA 23-436) (His tag)

Catalog No. ABIN1945405

Product Details

Target: SPOCK3 (Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 3 (SPOCK3))

Protein Type: Recombinant

Protein Characteristics: AA 23-436

Origin: Mouse

Source: Human Cells

Purification tag / Conjugate: This SPOCK3 protein is labelled with His tag.

Purpose: Recombinant Mouse Testican-3/SPOCK3 (C-6His)

Sequence: AAAVAVAGGR SDGGNFLDEK QWLTTISQYD KEVGQWNKFR DEVEDDYFRT WNPGKPFDQA LDPAKDPCLK TKCSRHKVCI TQDAQTALCI SHRRLTHSMK EVGGSHKQWR GLPSSTCKPC PIAYASPVCG SDGHSYSSQC KLEYQACVLG KQISIKCEGR CPCPSDKSMN IGRNVKRACS DLEFREVANR LRDWFKALHE SGSQNKKTKA LLRPERSRFD TSILPICKDS LGWMFNRLDT NYDLLLDQSE LGSIYLDKNE QCTKAFFNSC DTYKDSLISN NEWCYCFQRQ QDPPCHTELS NIQKRQGIKK LLGQYIPLCD EDGYYKPTQC HGSVGQCWCV DRYGNEVVGS RINGVADCAI DFEISGDFAS GDFREWTDDE GEEDDIMNDK DDIEDDDEDE GDDDDDGDVH DGYIVDHHHH HH

Characteristics: Recombinant Mouse Testican-3/SPOCK3 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (AA 23-436) of Mouse SPOCK3 fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SPOCK3 (Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 3 (SPOCK3))

Alternative Name: SPOCK3 (SPOCK3 Products)

Synonyms: im:7139568 Protein, si:dkey-285b22.1 Protein, HSAJ1454 Protein, TES-3 Protein, TICN3 Protein, Testican-3 Protein, 2900045C01Rik Protein, AI428471 Protein, mKIAA4039 Protein, sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3 Protein, SPARC/osteonectin, cwcv and kazal like domains proteoglycan 3 Protein, sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3 Protein, spock3 Protein, SPOCK3 Protein, Spock3 Protein

Background: SPOCK3，also called Testican3,is a secreted protein and make up a family of extracellular heparan/chondroitin sulfate proteoglycans. It contains 1 Kazal-like domain and 1 thyroglobulin type-1 domain and mainly expressed in brain. SPOCK3 contain inhibitory regions in several domains targeted to different classes of protease, and in some cases may act as protease inhibitors. In addition to their presence in testis, testicans are enriched in brain and have been shown to regulate neuronal attachment and outgrowth.

Molecular Weight: 47.9 kDa

Pathways: Glycosaminoglycan Metabolic Process