PFKFB1 Protein (AA 2-471) (His tag)
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- Target See all PFKFB1 Proteins
- PFKFB1 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-471
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This PFKFB1 protein is labelled with His tag.
- Purpose
- Recombinant Human Fructose-2,6-Bisphosphatase 1/PFKFB1/PFK/FBPase 1 (C-6His)
- Sequence
- SPEMGELTQT RLQKIWIPHS SGSSRLQRRR GSSIPQFTNS PTMVIMVGLP ARGKTYISTK LTRYLNWIGT PTKVFNLGQY RREAVSYKNY EFFLPDNMEA LQIRKQCALA ALKDVHNYLS HEEGHVAVFD ATNTTRERRS LILQFAKEHG YKVFFIESIC NDPGIIAENI RQVKLGSPDY IDCDREKVLE DFLKRIECYE VNYQPLDEEL DSHLSYIKIF DVGTRYMVNR VQDHIQSRTV YYLMNIHVTP RSIYLCRHGE SELNIRGRIG GDSGLSVRGK QYAYALANFI QSQGISSLKV WTSHMKRTIQ TAEALGVPYE QWKALNEIDA GVCEEMTYEE IQEHYPEEFA LRDQDKYRYR YPKGESYEDL VQRLEPVIME LERQENVLVI CHQAVMRCLL AYFLDKSSDE LPYLKCPLHT VLKLTPVAYG CKVESIYLNV EAVNTHREKP ENVDITREPE EALDTVPAHY VDHHHHHH
- Characteristics
- Recombinant Human PFKFB1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (AA 2-471) of Human PFKFB1 fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product PFKFB1 Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- PFKFB1 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1))
- Alternative Name
- PFKFB1 (PFKFB1 Products)
- Synonyms
- zgc:55328 Protein, wu:fi11h10 Protein, PFKFB1 Protein, F6PK Protein, HL2K Protein, PFRX Protein, Pfkfb01 Protein, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 Protein, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 S homeolog Protein, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 Protein, pfkfb1 Protein, PFKFB1 Protein, pfkfb1.S Protein, LOC100475853 Protein, Pfkfb1 Protein
- Background
- 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 is an enzyme that in humans is encoded by the PFKFB1 gene. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. It belongs to the phosphoglycerate mutase family. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis.
- Molecular Weight
- 55.6 kDa
- UniProt
- P16118
- Pathways
- Regulation of Carbohydrate Metabolic Process
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