NME/NM23 Nucleoside Diphosphate Kinase 3 (NME3) (AA 22-169) (Active) protein (His tag)

Details for Product No. ABIN1981651
Protein Name
Protein Characteristics
AA 22-169
3
2
1
1
Origin
Human
6
1
1
Source
Escherichia coli (E. coli)
5
2
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
His tag
Application
SDS-PAGE (SDS)
Options
Sequence MGSSHHHHHH SSGLVPRGSH MERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPV VAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV ESARREIALW FRADELLCWE DSAGHWLYE
Characteristics Molecule Information: 19.1 kDa (169 aa, 22-169 aa + His Tag)
Purity ≥95 % by SDS-PAGE
Biological Activity Comment Specific activity is > 20 units/ml, in which one unit will convert 1.0 μmole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25°C in a coupled system with PK/LDH.
Unit Definition One unit will convert 1.0 μmole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25°C in a coupled system with PK/LDH.
Protein Name
Background NME3, also known as, a potential suppressor of metastasis, is expressed at a much lower level in highly metastatic cells than in cells with lower metastatic potential. It is important for the synthesis of nucleoside triphosphates and may play a role in apoptosis induction and hematopoiesis. It is preferentially expressed during early stages of myeloid differentiation of highly purified CD34+ cells. Recombinant human NME3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternative Names: Nucleoside diphosphate kinase 3, c371H6.2, DR-nm23, KIAA0516, NDPK-C, NDPKC, NM23-H3, NM23H3.
Molecular Weight 19.1 kDa
Gene ID 4832
UniProt Q13232
Pathways Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.5 mg/mL solution in 20 mM Tris-HCl buffer ( pH 8.0) containing 50 % glycerol, 0.1 M NaCl and 2 mM DTT
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Centrifuge the vial prior to opening.
Storage -20 °C
Expiry Date 12 months
Supplier Images
Image no. 1 for NME/NM23 Nucleoside Diphosphate Kinase 3 (NME3) (AA 22-169) (Active) protein (His tag) (ABIN1981651) NME/NM23 Nucleoside Diphosphate Kinase 3 (NME3) (AA 22-169) (Active) protein (His tag)