Phosphoglycerate Kinase 2 (PGK2) (AA 1-417) (Active) protein (His tag)

Details for Product No. ABIN1981682, Supplier: Log in to see
Protein Name
  • PGKB
  • PGKPS
  • dJ417L20.2
  • Pgk-2
  • Tcp-2
  • PGK2
  • phosphoglycerate kinase 2
  • PGK2
  • Pgk2
  • cbbK
Protein Characteristics
AA 1-417
6
2
1
1
1
Origin
Human
7
1
1
1
1
Source
Escherichia coli (E. coli)
3
2
2
2
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
His tag
Application
SDS-PAGE (SDS)
Options
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Sequence MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM
Characteristics Molecule Information: 46.9 kDa (437 aa, 1-417 aa + His Tag)
Purity ≥85 % by SDS-PAGE
Biological Activity Comment Specific activity is >11 units/mg. One unit will convert 1.0 μmole of beta-NADH per minute in a coupled system with GAPDH at pH 8.0 at 25°C
Unit Definition One unit will convert 1.0 μmole of beta-NADH per minute in a coupled system with GAPDH at pH 8.0 at 25°C
Background PGK2 is a testis-specific form of phosphoglycerate kinase. Initially assumed to be a pseudogene, this protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1, 3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis. Recombinant human PGK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternative Names: Phosphoglycerate kinase 2, dJ417L20.2, PGKB, PGKPSS
Molecular Weight 46.9 kDa
Gene ID 5232
UniProt P07205
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.5 mg/mL solution in 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 0.1 M NaCl and 1 mM DTT
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Centrifuge the vial prior to opening.
Storage -20 °C
Expiry Date 12 months
Supplier Images
SDS-PAGE (SDS) image for Phosphoglycerate Kinase 2 (PGK2) (AA 1-417) (Active) protein (His tag) (ABIN1981682) Phosphoglycerate Kinase 2 (PGK2) (AA 1-417) (Active) protein (His tag)
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