Oligodendrocyte Myelin Glycoprotein (OMG) (AA 1-420), (Mature) (Active) protein (His tag)

Details for Product No. ABIN2002478
Protein Name
Protein Characteristics
AA 1-420, Mature
2
1
1
1
1
1
1
1
Origin
Human
7
3
Source
Human Cells
2
2
2
2
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
This OMG protein is labelled with His tag.
Application
Functional Studies (Func), SDS-PAGE (SDS)
Options
Characteristics The secreted recombinant human OMGP comprises 407 amino acids and has a predicted molecular mass of 46 kDa. The apparent molecular mass of rh OMGP is approximately 120-130 kDa in SDS-PAGE under reducing conditions due to high glycosylation.

Protein Structure: A DNA sequence encoding the mature form of human OMGP (P23515-1) (Met 1-Asn 420) without the pro peptide was expressed, fused with a polyhistidine tag at the C-terminus.
Predicted N-Term: Ile 25
Purity > 97 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells . Immobilized OMG (0.8 μg/ml, 100 μl/well) will mediate >20% C6 cell adhesion.
Protein Name
Background Synonyms: OMGP
Molecular Weight 46 kDa, 120-130 kDa
Pathways Neurotrophin Signaling Pathway, Regulation of Cell Size
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months