Complement Component 5 (C5) (AA 679-751), (active) (Active) Protein

Details for Product No. ABIN2003124
Protein Name
Protein Characteristics
AA 679-751, active
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Origin
Human
10
7
4
1
1
1
1
Source
Escherichia coli (E. coli)
8
6
6
3
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
Functional Studies (Func), SDS-PAGE (SDS)
Options
Characteristics The recombinant human C5a consists of 74 amino acids and has a predicted molecular mass of 8.3 kDa which is also estimated by SDS-PAGE.

Protein Structure: A DNA sequence encoding the active form of human C5a (Leu 679-Arg 751) was expressed.
Predicted N-Term: Based on N-terminal sequencing analysis, the first 7 aa of C5a is: MLQKKIE
Purity > 94 % as determined by SDS-PAGE
Biological Activity Comment Measured by its ability to induce N-acetyl-β-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. The ED50 for this effect is typically 5-15 ng/ml.
Protein Name
Background Synonyms: C5a,C5b,C5D,C5Da,Complement 5a,CPAMD4,ECLZB
Molecular Weight 8.3 kDa
Pathways Complement System, Carbohydrate Homeostasis
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months
Product cited in: Seow, Lim, Iyer, Suen, Ariffin, Hohenhaus, Sweet, Fairlie: "Inflammatory responses induced by lipopolysaccharide are amplified in primary human monocytes but suppressed in macrophages by complement protein C5a." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 8, pp. 4308-16, 2013 (PubMed).