Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (AA 2-1058) protein (GST tag,His tag)

Details for Product No. ABIN2004962
Protein Name
Protein Characteristics
AA 2-1058
1
1
1
1
Origin
Human
5
Source
Baculovirus infected Insect Cells
2
1
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This UBA1 protein is labelled with GST tag,His tag.
Application
SDS-PAGE (SDS)
Options
Characteristics The recombinant human UBA1/GST chimera consists of 1294 amino acids and has a calculated molecular mass of 146 KDa. It migrates as an approximately 130 KDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human UBA1 (NP_003325.2) (Ser 2-Arg 1058) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Predicted N-Term: Met
Purity > 96 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
Protein Name
Background Synonyms: A1S9,A1S9T,A1ST,AMCX1,CFAP124,CTD-2522E6.1,GXP1,POC20,SMAX2,UBA1A,UBE1,UBE1X
NCBI Accession NP_003325
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 7.4, 10 % gly, 0.5 mM GSH
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months