Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 2-317) protein (His tag)

Details for Product No. ABIN2004994, Supplier: Log in to see
Protein Name
  • AI462015
  • Nfkbi
  • IKBA
  • MAD-3
  • NFKBI
  • ECI-6/IKBA
  • RL/IF-1
  • NFKB inhibitor alpha
  • nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha
  • NFKBIA
  • Nfkbia
Protein Characteristics
AA 2-317
3
2
1
1
1
Origin
Human
7
1
1
Source
Escherichia coli (E. coli)
4
2
1
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This NFKBIA protein is labelled with His tag.
Application
SDS-PAGE (SDS)
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Characteristics The recombinant human NFKBIA consisting of 323 amino acids and has a calculated molecular mass of 36.4 kDa. It migrates as an approximately 38 kDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human NFKBIA (NP_065390.1) (Phe 2-Leu 317) was expressed, with a polyhistide tag at the N-terminus.
Predicted N-Term: Met
Purity > 90 % as determined by SDS-PAGE
Protein Name Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA)
Background Synonyms: IKBA,MAD-3,NFKBI
Molecular Weight 36.4 kDa, 38 kDa
NCBI Accession NP_065390
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile 50 mM Tris, 0.5M NaCl, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months
Supplier Images
 image for Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 2-317) protein (His tag) (ABIN2004994) Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 2-317) protein (His tag)
Product cited in: Brobeil, Kämmerer, Tag, Steger, Gattenlöhner, Wimmer: "PTPIP51—A New RelA-tionship with the NF?B Signaling Pathway." in: Biomolecules, Vol. 5, Issue 2, pp. 485-504, 2015 (PubMed).

Background publications Courtois, Smahi, Reichenbach, Döffinger, Cancrini, Bonnet, Puel, Chable-Bessia, Yamaoka, Feinberg, Dupuis-Girod, Bodemer, Livadiotti, Novelli, Rossi, Fischer, Israël, Munnich, Le Deist, Casanova: "A hypermorphic IkappaBalpha mutation is associated with autosomal dominant anhidrotic ectodermal dysplasia and T cell immunodeficiency." in: The Journal of clinical investigation, Vol. 112, Issue 7, pp. 1108-15, 2003

Courtois, Smahi, Reichenbach, Döffinger, Cancrini, Bonnet, Puel, Chable-Bessia, Yamaoka, Feinberg, Dupuis-Girod, Bodemer, Livadiotti, Novelli, Rossi, Fischer, Israël, Munnich, Le Deist, Casanova: "A hypermorphic IkappaBalpha mutation is associated with autosomal dominant anhidrotic ectodermal dysplasia and T cell immunodeficiency." in: The Journal of clinical investigation, Vol. 112, Issue 7, pp. 1108-15, 2003 (PubMed).

Ota, Suzuki, Nishikawa, Otsuki, Sugiyama, Irie, Wakamatsu, Hayashi, Sato, Nagai, Kimura, Makita, Sekine, Obayashi, Nishi, Shibahara, Tanaka, Ishii, Yamamoto, Saito, Kawai, Isono, Nakamura, Nagahari et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Jungnickel, Staratschek-Jox, Bräuninger, Spieker, Wolf, Diehl, Hansmann, Rajewsky, Küppers: "Clonal deleterious mutations in the IkappaBalpha gene in the malignant cells in Hodgkin's lymphoma." in: The Journal of experimental medicine, Vol. 191, Issue 2, pp. 395-402, 2000 (PubMed).

Scherer, Brockman, Chen, Maniatis, Ballard: "Signal-induced degradation of I kappa B alpha requires site-specific ubiquitination." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 24, pp. 11259-63, 1995 (PubMed).

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