Pepsinogen 4, Group I (Pepsinogen A) (PGA4) (AA 1-388) (Active) protein (Fc Tag)

Details for Product No. ABIN2005412
Protein Name
Protein Characteristics
AA 1-388
2
1
Origin
Human
4
Source
Human Cells
2
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
This PGA4 protein is labelled with Fc Tag.
Application
Functional Studies (Func), SDS-PAGE (SDS)
Options
Characteristics The secreted recombinant human PGA4/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 614 amino acids and has a predicted molecular mass of 67.3 kDa. The apparent molecular mass of rhPGA/Fc monomer is approximately 65-70 kDa in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human PGA4 (P00790) (Met 1-Ala 388) was fused with the Fc region of human IgG1 at the C-terminus.
Predicted N-Term: Ile 16
Purity > 80 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2, AnaSpec, Catalog # 27114.
The specific activity is > 3000 pmoles/min/μg.
(Activation description: The enzyme achieves its activity under acidic pH)
Protein Name
Background Synonyms: FLJ58952,FLJ77962,PGA3,PGA4,PGA5
Molecular Weight 67.3 kDa, 65-70 kDa
UniProt P00790
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months