Pepsinogen 4, Group I (Pepsinogen A) (PGA4) (AA 1-388) (Active) protein (His tag)

Details for Product No. ABIN2005414
Protein Name
Protein Characteristics
AA 1-388
Human Cells
Protein Type
Biological Activity
Purification tag / Conjugate
This PGA4 protein is labelled with His tag.
Functional Studies (Func), SDS-PAGE (SDS)
Characteristics The secreted recombinant human PGA4 consists of 384 amino acids and has a predicted molecular mass of 41.7 kDa. It migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human PGA4 (P00790) (Met 1-Ala 388) was fused with a polyhistidine tag at the C-terminus.
Predicted N-Term: Ile 16
Purity > 97 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2, AnaSpec, Catalog # 27114.
The specific activity is > 10,000 pmoles/min/μg.
(Activation description: The enzyme achieves its activity under acidic pH)
Protein Name
Background Synonyms: FLJ58952,FLJ77962,PGA3,PGA4,PGA5
Molecular Weight 41.7 kDa, 45 kDa
UniProt P00790
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months