Insulin-Like Growth Factor Binding Protein 7 (IGFBP7) (AA 1-282) (Active) protein (His tag)

Details for Product No. ABIN2005452
Protein Name
  • zgc:85888
  • AGM
  • FSTL2
  • IBP-7
  • IGFBP-7
  • IGFBP-7v
  • IGFBPRP1
  • MAC25
  • PSF
  • RAMSVPS
  • TAF
  • Fstl2
  • Mac25
  • insulin like growth factor binding protein 7
  • insulin-like growth factor binding protein 7
  • IGFBP7
  • igfbp7
  • Igfbp7
Protein Characteristics
AA 1-282
3
2
2
2
1
1
1
Origin
Human
19
5
2
1
Source
Human Cells
15
6
4
2
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
His tag
Application
Functional Studies (Func), SDS-PAGE (SDS)
Options
Characteristics The secreted recombinant human IGFBP7 comprises 267 amino acids and has a predicted molecular mass of 27.9 kDa. Since IGFBP7 can be proteolytically cleaved between lysine 97 and alanine 98, the apparent molecular mass of rh IGFBP7 is approximately 36 & 32 kDa in SDS-PAGE under reducing conditions, corresponding to the whole protein and the cleaved form respectively.

Protein Structure: A DNA sequence encoding the human IGFBP7 (Q16270) (Met 1-Leu 282) was fused with a polyhistidine tag at the C-terminus.
Predicted N-Term: Ser 27
Purity > 92 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its binding ability in a functional ELISA. Immobilized IGFBP7 at 20 μg/ml (100 μl/well) can bind biotinylated human IGF2-nusa . The EC50 of biotinylated human IGF2-nusa is 0.57 μg/ml.
Protein Name
Background Synonyms: AGM,FSTL2,IBP-7,IGFBP-7,IGFBP-7v,IGFBPRP1,MAC25,PSF,RAMSVPS,TAF
Molecular Weight 27.9 kDa, 32 kDa
UniProt Q16270
Pathways Growth Factor Binding
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months
Background publications Gommans, Tatalias, Sie, Dupuis, Vendetti, Smith, Kaushal, Maas: "Screening of human SNP database identifies recoding sites of A-to-I RNA editing." in: RNA (New York, N.Y.), Vol. 14, Issue 10, pp. 2074-85, 2008 (PubMed).

Ahmed, Jin, Yagi, Yasuda, Sato, Higashi, Lin, Dickson, Miyazaki: "Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25)." in: The FEBS journal, Vol. 273, Issue 3, pp. 615-27, 2006 (PubMed).

Wilson, Oh, Hwa, Rosenfeld: "Interaction of IGF-binding protein-related protein 1 with a novel protein, neuroendocrine differentiation factor, results in neuroendocrine differentiation of prostate cancer cells." in: The Journal of clinical endocrinology and metabolism, Vol. 86, Issue 9, pp. 4504-11, 2001 (PubMed).

Oh, Nagalla, Yamanaka, Kim, Wilson, Rosenfeld: "Synthesis and characterization of insulin-like growth factor-binding protein (IGFBP)-7. Recombinant human mac25 protein specifically binds IGF-I and -II." in: The Journal of biological chemistry, Vol. 271, Issue 48, pp. 30322-5, 1997 (PubMed).

Akaogi, Okabe, Funahashi, Yoshitake, Nishikawa, Yasumitsu, Umeda, Miyazaki: "Cell adhesion activity of a 30-kDa major secreted protein from human bladder carcinoma cells." in: Biochemical and biophysical research communications, Vol. 198, Issue 3, pp. 1046-53, 1994 (PubMed).

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