Acetylcholinesterase (AChE) (AA 1-614) (Active) protein (His tag) Protein
AChE Origin: Mouse Source: Human Cells Recombinant > 97 % as determined by SDS-PAGE Func, SDS
Catalog No. ABIN2007952
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- Protein Name
- Protein Type
- Biological Activity
- Protein Characteristics
- AA 1-614
- Human Cells
- Purification tag / Conjugate
- This Acetylcholinesterase protein is labelled with His tag.
- Functional Studies (Func), SDS-PAGE (SDS)
- The recombinant mouse ACHE consists of 594 amino acids and has a predicted molecular mass of 66.2 kDa as estimated in SDS-PAGE under reducing conditions.
Protein Structure: A DNA sequence encoding the mouse ACHE (NP_033729.1) (Met 1-Leu 614) was expressed, with a polyhistidine tag at the C-terminus.
Predicted N-Term: Glu 32
- > 97 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method
- Biological Activity Comment
- Measured by its ability to cleave Acetylthiocholine.
The specific activity is > 250 nmols/min/μg.
- Application Notes
- Optimal working dilution should be determined by the investigator.
- For Research Use only
- Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
- Handling Advice
- Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
- -20 °C,-80 °C
- Storage Comment
- Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
- Expiry Date
- 12 months
Sperling, Klaczinski, Schütz, Rudolph, Layer: "Mouse acetylcholinesterase enhances neurite outgrowth of rat R28 cells through interaction with laminin-1." in: PLoS ONE, Vol. 7, Issue 5, pp. e36683, 2012 (PubMed).
- Sperling, Klaczinski, Schütz, Rudolph, Layer: "Mouse acetylcholinesterase enhances neurite outgrowth of rat R28 cells through interaction with laminin-1." in: PLoS ONE, Vol. 7, Issue 5, pp. e36683, 2012 (PubMed).
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