Epigen Protein (AA 24-95)
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- Target See all Epigen (EPGN) Proteins
- Epigen (EPGN) (Epithelial Mitogen (EPGN))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 24-95
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Characteristics
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ED50 < 1 xg/mL, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 1x 10^3 units/mg.
AA 24-95, expressed with an N-terminal Met. - Purity
- > 95 % by SDS-PAGE analysis.
- Endotoxin Level
- < 0.2 EU/μg, determined by LAL method.
- Top Product
- Discover our top product EPGN Protein
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Epithelial Mitogen (EPGN) (AA 24-95) protein (His tag)
EPGN Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Epithelial Mitogen (EPGN) (AA 1-133) protein (GST tag)EPGN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Epithelial Mitogen (EPGN) (AA 23-154) protein (rho-1D4 tag)Crystallography grade EPGN Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Epithelial Mitogen (EPGN) (AA 19-152) protein (rho-1D4 tag)Crystallography grade EPGN Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Epithelial Mitogen (EPGN) (AA 23-110) protein (His tag)EPGN Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % by SDS - PAGE SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstituted in ddH2O at 100 μg/mL.
- Buffer
- Lyophilized after extensive dialysis against PBS.
- Storage
- -80 °C
- Storage Comment
- Lyophilized recombinant human Epigen (rhEpigen) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhEpigen remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
- Expiry Date
- 6 months
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- Target
- Epigen (EPGN) (Epithelial Mitogen (EPGN))
- Alternative Name
- Epigen (EPGN Products)
- Synonyms
- ALGV3072 Protein, EPG Protein, PRO9904 Protein, 2310069M11Rik Protein, epigen Protein, RGD1560084 Protein, epithelial mitogen Protein, EPGN Protein, Epgn Protein
- Background
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Epigen is a cytokine belonging to the Epidermal Growth Factor (EGF) superfamily, which also includes Epiregulin, Amphiregulin, Neuregulin 2-beta, and Transforming Growth Factor alpha. The precursor of Epigen produced in tissues has 154 amino acids, and shares the characteristics of other members of EGF superfamily, including 3 disulfide bonds formed by 6 cysteines. Epigen is present in testis, heart, and liver, and it binds to EGF receptors with a much lower binding affinity than EGF. However, Epigen is more mitogenic than EGF. Epigen achieves its strong mitogenic potency by suppressing ligand-induced receptor inactivation. Unlike EGF, Epigen can also bind to EGF receptors in low pH conditions, helping its recycling. Therefore Epigen has anomalous potency due to its prolonged presence.Recombinant human Epigen (rhEpigen) produced in E. coli is a single non-glycosylated polypeptide chain containing 73 amino acids. A fully biologically active molecule, rhEpigen has a molecular mass of 8.1 kDa analyzed by reducing SDS-PAGE.
Synonyms: EPG, Epithelial mitogen - Molecular Weight
- 8.1 kDa, observed by reducing SDS-PAGE.
- Pathways
- RTK Signaling, EGFR Signaling Pathway
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