IL-10 Protein (AA 19-178, Arg25Lys-Mutant, Leu65Val-Mutant)

Catalog No. ABIN2018042

Product Details

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 19-178, Arg25Lys-Mutant, Leu65Val-Mutant

Origin: Rat

Source: Escherichia coli (E. coli)

Characteristics: ED50 < 0.5 μg/mL, measured by a cell proliferation assay using C6 cells, corresponding to a specific activity of > 2.0x 10^3 units/mg.
AA 19-178 (Arg25Lys, Leu65Val), expressed with an N-terminal Met.

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant rat Interleukin-10 (IL-10) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, IL-10 should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: Interleukin-10 (IL-10) (IL10 Products)

Synonyms: CSIF Protein, GVHDS Protein, IL-10 Protein, IL10A Protein, TGIF Protein, Il-10 Protein, IL10X Protein, interleukin 10 Protein, IL10 Protein, Il10 Protein

Background: Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine produced by a variety of cell lines including T-cells, macrophages and mast cells. IL-10 is classified as a class-2 cytokine, a set of cytokines including IL-19, IL-20, IL-22, IL-24, and IL-26. IL-10 can inhibit the synthesis of pro-inflammatory cytokines such as IFN-gamma, IL-2, IL-3, TNF and GM-CSF. It also stimulates Th2 responses, but suppresses the antigen-presentation capacity of antigen presenting cells.Recombinant rat Interleukin-10 (IL-10)produced in E. coli is a single non-glycosylated polypeptide chain containing 161 amino acids. A fully biologically active molecule, recombinant rat Interleukin-10 (IL-10) has a molecular mass of 18.7 kDa analyzed by reducing SDS-PAGE.
Synonyms: Cytokine synthesis inhibitory factor, CSIF, TGIF, IL10A

Molecular Weight: 18.7 kDa, observed by reducing SDS-PAGE.

UniProt: P29456

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints