LIF Protein (AA 23-202)

Catalog No. ABIN2018194

Product Details

Target: LIF (Leukemia Inhibitory Factor (LIF))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 23-202

Origin: Human

Source: Escherichia coli (E. coli)

Characteristics: ED50 < 0.2 ng/mL, measured by a cell differentiation assay using TF-I cells, corresponding to a specific activity of > 5 x 10^6 units/mg.

Purity: > 95 % by SDS-PAGE analysis.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Leukemia Inhibitory Factor (rhLIF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhLIF remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C. ,

Expiry Date: 6 months

References

Huang, Li, Alonso-Gonzalez, Gorre, Keatley, Green, Turner, Kallingappa, Verma, Oback: "A virus-free poly-promoter vector induces pluripotency in quiescent bovine cells under chemically defined conditions of dual kinase inhibition." in: PLoS ONE, Vol. 6, Issue 9, pp. e24501, (2011) (PubMed).

Yu, Kastin, Pan: "TNF reduces LIF endocytosis despite increasing NFkappaB-mediated gp130 expression." in: Journal of cellular physiology, Vol. 213, Issue 1, pp. 161-6, (2007) (PubMed).

Target Details

Target: LIF (Leukemia Inhibitory Factor (LIF))

Abstract: LIF Products

Synonyms: CDF Protein, DIA Protein, HILDA Protein, MLPLI Protein, LIF, interleukin 6 family cytokine Protein, leukemia inhibitory factor Protein, LIF Protein, Lif Protein

Background: Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine belonging to the long four-helix bundle cytokine superfamily. LIF shares tertiary structure with several other cytokines, including Interleukin-6 (IL-6), Oncostatin M, ciliary neurotropic factor, and cardiotrophin-1, and their functions in vivo are also redundant to some extent. LIF can bind to the common receptor of IL-6 subfamily, gp130, and then recruit its own receptor LIF Receptor to form a ternary complex. The basal expression of LIF in vivo is low, and its expression is induced by pro-inflammatory factors, including lipopolysaccharide, IL-1, and IL-17, and inhibited by anti-inflammatory agents, including IL-4 and IL-13. The functions of LIF include proliferation of primordial germ cells, regulation in blastocyst implantation and early pregnancy, and maintenance of pluripotent embryonic stem cells.Recombinant human Leukemia Inhibitory Factor (rhLIF) produced in E. coli is a single non-glycosylated polypeptide chain containing 180 amino acids. A fully biologically active molecule, rhLIF has a molecular mass of 19.7 kDa analyzed by reducing SDS-PAGE.
Synonyms: D factor, MLPLI

Molecular Weight: 19.7 kDa, observed by reducing SDS-PAGE.

UniProt: P15018

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding