YY1 Protein (AA 221-321) (His tag)

Catalog No. ABIN2115531

Product Details

Target: YY1 (YY1 Transcription Factor (YY1))

Protein Type: Recombinant

Protein Characteristics: AA 221-321

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This YY1 protein is labelled with His tag.

Purpose: Recombinant Human Transcriptional Repressor Protein YY1 (C-6His)

Sequence: MVTMWSSDEK KDIDHETVVE EQIIGENSPP DYSEYMTGKK LPPGGIPGID LSDPKQLAEF ARMKPRKIKE DDAPRTIACP HKGCTKMFRD NSAMRKHLHT HGLEHHHHHH

Characteristics: Recombinant Human Transcriptional repressor protein YY1 is produced with our E. coli expression system. The target protein is expressed with sequence (Val221-Gly321) of Human YY1 fused with a 6His tag at the C-terminus..

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: YY1 (YY1 Transcription Factor (YY1))

Alternative Name: YY1 (YY1 Products)

Synonyms: yy1-a Protein, FIII Protein, MGC83714 Protein, yy1l Protein, zgc:64207 Protein, YY1 Protein, LOC494431 Protein, xyy1 Protein, delta Protein, nf-e1 Protein, ucrbp Protein, yin-yang-1 Protein, LOC100304682 Protein, ino80s Protein, yy1 Protein, DELTA Protein, INO80S Protein, NF-E1 Protein, UCRBP Protein, YIN-YANG-1 Protein, AW488674 Protein, fb59g10 Protein, wu:fa16g07 Protein, wu:fb59g10 Protein, YY1 transcription factor L homeolog Protein, YY1 transcription factor b Protein, YY1 transcription factor Protein, transcriptional repressor protein YY1 Protein, YY1 transcription factor S homeolog Protein, YY1 transcription factor a Protein, YY2 transcription factor Protein, yy1.L Protein, yy1b Protein, YY1 Protein, yy1 Protein, LOC100304682 Protein, yy1.S Protein, Yy1 Protein, yy1a Protein, YY2 Protein

Background: Transcriptional repressor protein YY1(YY1)contains 4 C2H2-type zinc fingers and belongs to the YY transcription factor family. Multifunctional transcription factor exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. The effect on transcription regulation of the protein is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression.
Synonyms: Transcriptional repressor protein YY1,Delta transcription factor,INO80 complex subunit S,NF-E1,Yin and yang 1,INO80S

Molecular Weight: 12.6 kDa

UniProt: P25490