Streptavidin protein (IRDye800CW)

Details for Product No. ABIN2169598, Supplier: Log in to see
Protein Name
Streptomyces avidinii (S. avidinii)
Streptomyces avidinii
Protein Type
Purification tag / Conjugate
This Streptavidin protein is labelled with IRDye800CW.
In-Gel Western blotting (gelWB), Light Microscopy (LM), Protein Array (PAr), ELISA, Western Blotting (WB)
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Specificity Biotin
Characteristics IRDye 800CW Streptavidin can be used as a secondary detection reagent for protein arrays, microscopy, In-Gel Western Assays, and Western blotting. To reduce non-specific background staining due to possible aggregates in the solution, centrifuge briefly before use. A final concentration of 0.2 to 1.0 μg/mL (1:1,000 to 1:5,000) is satisfactory for most applications, however, appropriate dilution may need to be determined empirically.
For membrane-based applications and In-Gel Westerns, it is recommended to add SDS (0.02 % to 0.1 % final concentration) and Tween 20 (0.1 to 0.2 % final concentration) during the detection incubation step to reduce non-specific background staining.
Research Area Epitope Tags, Tags/Labels, Enzymes
Application Notes Western blot: 1:1,000-1:5,000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 10 mM Phosphate, 183 mM NaCl, 2.7 mM KCl, pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light.
Storage 4 °C
Product cited in: Rios-Avila, Prince, Wijeratne, Zempleni: "A 96-well plate assay for high-throughput analysis of holocarboxylase synthetase activity." in: Clinica chimica acta; international journal of clinical chemistry, Vol. 412, Issue 9-10, pp. 735-9, 2011 (PubMed).

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