Poliovirus Receptor Protein (PVR) (AA 21-343) (His tag)

Catalog No. ABIN2180698

Product Details

Target: Poliovirus Receptor (PVR)

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 21-343

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Poliovirus Receptor protein is labelled with His tag.

Sequence: AA 21-343

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 28 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

References

Chandramohan, Bryant, Piao, Keir, Lipp, Lefaivre, Perkinson, Bigner, Gromeier, McLendon: "Validation of an Immunohistochemistry Assay for Detection of CD155, the Poliovirus Receptor, in Malignant Gliomas." in: Archives of pathology & laboratory medicine, Vol. 141, Issue 12, pp. 1697-1704, (2017) (PubMed).

Target Details

Target: Poliovirus Receptor (PVR)

Alternative Name: CD155 (PVR Products)

Synonyms: PVR Protein, CD155 Protein, HVED Protein, NECL5 Protein, Necl-5 Protein, PVS Protein, TAGE4 Protein, 3830421F03Rik Protein, D7Ertd458e Protein, Taa1 Protein, Tage4 Protein, mE4 Protein, necl-5 Protein, poliovirus receptor Protein, PVR Protein, Pvr Protein

Background: CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells. The fulllength (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N terminal V type and two C2 type Ig like domains. CD155 is a transmembrane protein with 3 extracellular immunoglobulin-like domains, D1-D3, where D1 is recognized by the virus. Low resolution structures of CD155 complexed with poliovirus have been obtained using electron microscopy while a high resolution structures of theectodomain D1 and D2 of CD155 were solved by x-ray crystallography.

Molecular Weight: 35.9 kDa

NCBI Accession: NP_006496

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Cancer Immune Checkpoints, SARS-CoV-2 Protein Interactome