FCER2 Protein (AA 48-321) (His tag)

Catalog No. ABIN2180728

Product Details

Target: FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))

Protein Type: Recombinant

Protein Characteristics: AA 48-321

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FCER2 protein is labelled with His tag.

Brand: MABSol®

Sequence: AA 48-321

Characteristics: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 32.6 kDa. The protein migrates as 40-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50  mM Tris, 150  mM NaCl, pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: 4-8°C for 1 year in lyophilized state 4-8°C for 1 month under sterile conditions after reconstitution -20°C to -70°C for 3 months under sterile conditions after reconstitution

Target Details

Target: FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))

Alternative Name: CD23 (FCER2 Products)

Synonyms: BLAST-2 Protein, CD23 Protein, CD23A Protein, CLEC4J Protein, FCE2 Protein, IGEBF Protein, Fcer2a Protein, Fc fragment of IgE receptor II Protein, FCER2 Protein, Fcer2 Protein

Background: Cluster of differentiation 23 (CD23) is also known as Low affinity immunoglobulin epsilon Fc receptor (FCER2), C-type lectin domain family 4 member J (CLEC4J), Fc-epsilon-RII (FcεRII), Immunoglobulin E-binding factor (IGEBF), is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. Unlike many of the antibody receptors, CD23 is a C-type lectin. It is found on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets.There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. CD23 is known to have role of transportation in antibody feedback regulation. Antigen that enters the blood stream is captured by antigen specific IgE antibodies. The IgE immune complexes that are formed bind to CD23 Molecules on B cells, and are transported to the B cell follicles of the spleen. The antigen is then transferred from CD23+ B cells to CD11c+ antigen presenting cells. The CD11c+ cells in turn present the antigen to CD4+ T cells, which can lead to an enhanced antibody response. In flow cytometry, CD23 is helpful in the differentiation of chronic lymphocytic leukemia (CD23-positive) from mantle cell leukemia (CD23-negative).

Molecular Weight: 32.9 kDa

NCBI Accession: NP_001993

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process