CD31 Protein (AA 28-601) (Fc Tag)

Catalog No. ABIN2181646

Product Details

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Protein Type: Recombinant

Protein Characteristics: AA 28-601

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD31 protein is labelled with Fc Tag.

Sequence: AA 28-601

Characteristics: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 90.5 kDa. The protein migrates as 100-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

References

Guzzo, Ichikawa, Park, Phillips, Liu, Zhang, Kwon, Miao, Lu, Rehm, Arthos, Cicala, Cohen, Fauci, Kehrl, Lusso: "Virion incorporation of integrin α4β7 facilitates HIV-1 infection and intestinal homing." in: Science immunology, Vol. 2, Issue 11, (2017) (PubMed).

Target Details

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31 (PECAM1 Products)

Synonyms: CD31 Protein, CD31/EndoCAM Protein, GPIIA' Protein, PECA1 Protein, PECAM-1 Protein, endoCAM Protein, C85791 Protein, Cd31 Protein, Pecam Protein, platelet/endothelial cell adhesion molecule 1 Protein, platelet and endothelial cell adhesion molecule 1 Protein, pecam1 Protein, PECAM1 Protein, Pecam1 Protein

Background: Cluster of Differentiation 31 (CD31) is also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a 130- kDa transmembrane glycoprotein expressed by endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, T / NK cells, lymphocytes, megakaryocytes, osteoclasts, neutrophils, certain tumors, and is the only known member of the CAM family on platelets. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. CD31 plays a key role in removing aged neutrophils from the body. CD31 mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development. In addition, it has been shown that CD31 expression is up-regulated by LPS stimulation, and might function as a feedback negative regulator of LPS inflammatory response in macrophages.

Molecular Weight: 90.6 kDa

Pathways: Regulation of Actin Filament Polymerization