DIABLO Protein (AA 56-239) (His tag)

Catalog No. ABIN2444099

Product Details

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Protein Type: Recombinant

Protein Characteristics: AA 56-239

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This DIABLO protein is labelled with His tag.

Sequence: AA 56-239

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.7 kDa. The protein migrates as 22 kDa under reducing (R) condition (SDS-PAGE).

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM HEPES, 150 mM NaCl, pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.

Target Details

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Alternative Name: SMAC (DIABLO Products)

Synonyms: smac Protein, smac3 Protein, diablo-s Protein, 0610041G12Rik Protein, 1700006L01Rik Protein, AU040403 Protein, Smac Protein, DFNA64 Protein, SMAC Protein, DBO Protein, XSmac Protein, diablo, IAP-binding mitochondrial protein Protein, diablo IAP-binding mitochondrial protein Protein, diablo, IAP-binding mitochondrial protein L homeolog Protein, diablo Protein, Diablo Protein, DIABLO Protein, diablo.L Protein

Background: Diablo homolog, mitochondrial (DIABLO) is also known as direct IAP-binding protein with low pI and second mitochondria-derived activator of caspase (SMAC), which can interact with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Acting by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP),DIABLO can also promote apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Also, DIABLO inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Furthermore, DIABLO is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.

Molecular Weight: 21.7 kDa

NCBI Accession: NP_063940

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity