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PCSK9 Protein (AA 31-692) (His tag,AVI tag,Biotin)

PCSK9 Origin: Human Host: HEK-293 Cells Recombinant >92 % as determined by reduced SDS-PAGE. Active
Catalog No. ABIN2444171
  • Target See all PCSK9 Proteins
    PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 31-692
    Origin
    • 18
    • 11
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    Human
    Source
    • 25
    • 7
    • 6
    • 2
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This PCSK9 protein is labelled with His tag,AVI tag,Biotin.
    Brand
    MABSol®,PrecisionAvi
    Sequence
    AA 31-692
    Specificity
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Characteristics
    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 72 kDa. The protein migrates as 18 kDa and 66 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and proteolytic digestion.
    Purity
    >92 % as determined by reduced SDS-PAGE.
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product PCSK9 Protein
  • Comment

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Handling Advice
    Please avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
  • Wang, Amiss, Weidmann, Craik: "Structure-activity analysis of truncated albumin-binding domains suggests new lead constructs for potential therapeutic delivery." in: The Journal of biological chemistry, Vol. 295, Issue 34, pp. 12143-12152, (2021) (PubMed).

    Chen, Lichtor, Berliner, Chen, Liu: "Evolution of sequence-defined highly functionalized nucleic acid polymers." in: Nature chemistry, Vol. 10, Issue 4, pp. 420-427, (2019) (PubMed).

    Wang, Ghani, Bundock, Weidmann, Harvey, Edwards, Schroeder, Swedberg, Craik: "Calcium-Mediated Allostery of the EGF Fold." in: ACS chemical biology, Vol. 13, Issue 6, pp. 1659-1667, (2019) (PubMed).

  • Target
    PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))
    Alternative Name
    PCSK9 (PCSK9 Products)
    Synonyms
    FH3 Protein, HCHOLA3 Protein, LDLCQ1 Protein, NARC-1 Protein, NARC1 Protein, PC9 Protein, AI415265 Protein, AI747682 Protein, Narc1 Protein, proprotein convertase subtilisin/kexin type 9 Protein, proprotein convertase subtilisin/kexin type 9 L homeolog Protein, PCSK9 Protein, pcsk9.L Protein, pcsk9 Protein, Pcsk9 Protein
    Background
    Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
    Molecular Weight
    13.8 kDa and 59.9 kDa
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