Protein L (Active) protein (Biotin,His tag)

Details for Product No. ABIN2444210, Supplier: Log in to see
Protein Name
Origin
Peptostreptococcus magnus (P. magnus)
5
2
1
1
1
1
1
1
1
Source
Escherichia coli (E. coli)
7
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
Biotin,His tag
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Brand MABSol®,UltraLys
Specificity The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Characteristics This protein carries a polyhistidine tag at the N-terminus. The protein migrates as 42-47 kDa on a SDS-PAGE gel under reducing (R) condition. This protein can bind to VL-Kappa.
Purity >95 % as determined by reduced SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Grade Ultra Sensitive
Background Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).
Comment

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for Protein L (Active) protein (Biotin,His tag) (ABIN2444210) Biotinylated Protein L, Superior Sensitivity on SDS-PAGE under reducing (R) condition...
Binding Studies (Bind) image for Protein L (Active) protein (Biotin,His tag) (ABIN2444210) Immobilized Human IgG1 containing Kappa L chain at 5 μg/mL (100 μL/well) can bind Bio...
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