RNASEH Protein
RNASEH
Origin: E. coli
Host: Escherichia coli (E. coli)
Recombinant
> 95 % protein determined by SDS-PAGE (CBB staining)
Active
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(3 references)-
- Target See all RNASEH Proteins
- RNASEH (Ribonuclease H (RNASEH))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- E. coli
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Source
- Escherichia coli (E. coli)
- Characteristics
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Specific Activity: 100, 000 units/mg protein
Unit Definition: 1 unit is defined as the amount of the enzyme that hydrolyzes 1 nmol of the RNA in 3H labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37 C. - Purity
- > 95 % protein determined by SDS-PAGE (CBB staining)
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- Application Notes
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1) Removal of mRNA in DNA/RNA hybrid prior to the synthesis of the second strand of cDNA.
2) Removal of poly (A) tails from mRNA after hybridization with oligo (dT)
3) Oligodeoxyribonucleotide-directed site-specific cleavage of RNA - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 50 units/L
- Buffer
- 50 units/ul in 20 mM Tris-HCl ( pH 7.5), 100 mM KCl, 1 mM DTT, 50 % Glycerol
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
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: "Second-strand cDNA synthesis: mRNA fragments as primers." in: Methods in enzymology, Vol. 152, pp. 330-5, (1987) (PubMed).
: "Site specific enzymatic cleavage of RNA." in: Nucleic acids research, Vol. 7, Issue 1, pp. 179-92, (1979) (PubMed).
: "Electrophoretic patterns of deadenylylated chorion and globin mRNAs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 72, Issue 8, pp. 2959-63, (1976) (PubMed).
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: "Second-strand cDNA synthesis: mRNA fragments as primers." in: Methods in enzymology, Vol. 152, pp. 330-5, (1987) (PubMed).
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- Target
- RNASEH (Ribonuclease H (RNASEH))
- Abstract
- RNASEH Products
- Synonyms
- BA1623 Protein, rnhA Protein, SC5H1.08c Protein, ribonuclease H Protein, ribonuclease HI Protein, Ribonuclease H Protein, rnhA Protein, SCO7284 Protein, CBU_0316 Protein, CNA03470 Protein, CNN02200 Protein, Tb10.70.2140 Protein, Tmath_1295 Protein, Deima_2376 Protein, Tresu_0232 Protein, Marky_0915 Protein, Desac_1125 Protein, Corgl_1288 Protein
- Background
- Ribonuclease H (RNase H) is an endoribonuclease which specifically degrades the RNA strand of an RNA/DNA hybrid, leaving the DNA strand and unhybridized RNA intact. E.coli RNase H (=RNaseHI) was over-expressed in E. coli as a recombinant protein and highly purified. MW is 17.6 kDa.
- UniProt
- P0A7Y4
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