FGF1 Protein

Catalog No. ABIN2452190

Product Details

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), Functional Studies (Func)

Characteristics: Product: Full length recombinant mature FGF-1 (15.8 kDa, 140 amino acids) expressed in E. coli. Activity: The ED50 as determined by a cell proliferation assay using MTS assay kit (Cell Titer 96, Promega) with Balb/c3T3 cells was < 10 pg/mL, corresponding to a specific activity of < 1 x 108 units/mg.

Purity: > 98 % as determined by SDS-PAGE (CBB staining)

Sterility: Sterile filtered

Application Details

Application Notes: 1. Use as a supplement in serum-free or reduced serum media for culture of mammalian cells
2. Studies of the human FGF-1 receptor, transmembrane signaling and protein phosphorylation
3. Western blotting control for anti-EGF-1 antibodies
4. Widely used in cosmetics,such as whitening,anti-wrinkle,anti-aging,etc.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2 mg/mL

Buffer: PBS (10 mM Na-phosphate, 150 mM NaCl) pH 7.2, 50 % glycerol

Storage: -20 °C/-80 °C

Storage Comment: -20 C (For long term storage: -70 C)

References

Zakrzewska, Marcinkowska, Wiedlocha: "FGF-1: from biology through engineering to potential medical applications." in: Critical reviews in clinical laboratory sciences, Vol. 45, Issue 1, pp. 91-135, (2008) (PubMed).

Target Details

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Alternative Name: Fibroblast Growth Factor 1 (FGF1 Products)

Synonyms: AFGF Protein, ECGF Protein, ECGF-beta Protein, ECGFA Protein, ECGFB Protein, FGF-1 Protein, FGF-alpha Protein, FGFA Protein, GLIO703 Protein, HBGF-1 Protein, HBGF1 Protein, Fgf1 Protein, FGF1 Protein, aFGF Protein, ecgf Protein, fgfa Protein, ecgfa Protein, ecgfb Protein, fgf-1 Protein, hbgf1 Protein, glio703 Protein, ecgf-beta Protein, fgf-alpha Protein, Dffrx Protein, Fam Protein, Fgf-1 Protein, Fgfa Protein, zgc:136885 Protein, LOC100221393 Protein, fgf1 Protein, zgc:73249 Protein, fibroblast growth factor 1 Protein, fibroblast growth factor 1 L homeolog Protein, fibroblast growth factor 1b Protein, fibroblast growth factor 1a Protein, FGF1 Protein, fgf1.L Protein, fgf1 Protein, Fgf1 Protein, fgf1b Protein, LOC100221393 Protein, fgf1a Protein

Background: FGF-1 (acidic FGF) is a member of the fibroblast growth factor (FGF) family, which binds heparin. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.

Gene ID: 2246

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway