Osteoprotegerin Protein (AA 22-401, C-Term)

Catalog No. ABIN2666865

Product Details

Target: Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 22-401, C-Term

Origin: Mouse

Source: HEK-293 Cells

Application: Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: ED50 = 30 - 90 ng/ml, corresponding to a specific activity of 1 - 3 x 104 units/mg, as determined by inhibition of cytotoxic effect of human TRAIL on L929 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Target Details

Target: Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))

Alternative Name: TNFRSF11B (TNFRSF11B Products)

Synonyms: CD254 Protein, ODF Protein, OPGL Protein, OPTB2 Protein, RANKL Protein, TRANCE Protein, hRANKL2 Protein, sOdf Protein, OCIF Protein, OPG Protein, TR1 Protein, Opg Protein, ocif Protein, opg Protein, tr1 Protein, TNF superfamily member 11 Protein, TNF receptor superfamily member 11b Protein, tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin) Protein, TNF receptor superfamily member 11B Protein, tumor necrosis factor receptor superfamily member 11b S homeolog Protein, tumor necrosis factor receptor superfamily, member 11b Protein, TNFSF11 Protein, TNFRSF11B Protein, Tnfrsf11b Protein, tnfrsf11b.S Protein

Target Type: Chemical

Background: TNFRSF11B is a member of the TNFR family, which lacks the transmembrane domain and acts as a secreted decoy receptor with no direct signaling capacity. It plays a key role in the physiological regulation of osteoclastic bone resorption, postnatal bone formation, and pathological calcification of arteries. TNFRSF11B binds to its natural ligand, RANKL (OPGL), which prevents the interaction of RANKL with its receptor, present on osteoclasts, therefore inhibiting osteoclast differentiation, activation, and survival. Overexpression of TNFRSF11B in transgenic mice leads to deep osteoporosis secondary to an almost total lack of osteoclasts. In addition, TNFRSF11B-deficient mice develop early onset osteoporosis and calcification of the aorta and renal arteries, sites which have endogenous TNFRSF11B expression in normal animals. EBF family member, EBF2 activates TNFRSF11B gene transcription in a synergistic manner with Wnt/β-catenin signaling transcription factor LEF1. In addition to RANKL, TNFRSF11B binds and neutralizes the pro-apoptotic actions of TNF-related apoptosis-inducing ligand (TRAIL) expressed by vascular smooth muscle cells (VSMCs) and T cells. Lastly, TNFRSF11B is involved in B cell development and function. High levels of TNFRSF11B have been detected in serum of patients with cardiovascular diseases, diabetes, and chronic renal failure.

Molecular Weight: The 620 amino acid recombinant protein has a predicted molecular mass of approximately 70.6 kDa. The DTT-reduced and non-reduced protein migrate at approximately 100 kDa and 200 kDa, respectively, by SDS-PAGE. The predicted N-terminal amino acid is Glu.