BRCA1 Protein (His tag)

Catalog No. ABIN2669409

Product Details

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus

Purification tag / Conjugate: This BRCA1 protein is labelled with His tag.

Application: In vitro Assay (in vitro), Proliferation Assay (ProA), Protein Interaction (PI)

Characteristics: The wild-type BRCA1 protein (1-1863 residues, accession number NM_007294) was expressed in a baculovirus system with an amino terminal polyhistidine tag and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 95 % homogeneous and contains no detectable protease, DNase and RNase activity.

Purification: Purified by an affinity column in combination with FPLC chromatography.

Purity: The purified recombinant protein is greater than 95 % homogeneous and contains no detectable protease, DNase and RNase activity.

Application Details

Application Notes: Recombinant BRCA1 is suitable for in vitro function studies including transcription and DNA repair, for protein-protein interaction assays and cell growth assays. 1 ng is sufficient for a gelshift assay in a 20 μL reaction volume, 50 ng is sufficient for reconstituted transcription assays and 100 ng is sufficient for protein-protein interaction studies. The molecular weight of the protein is ~205 kDa. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.

Restrictions: For Research Use only

Handling

Concentration: 0.4 μg/μL

Target Details

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1 (BRCA1 Products)

Synonyms: BRCAI Protein, BRCC1 Protein, BROVCA1 Protein, IRIS Protein, PNCA4 Protein, PPP1R53 Protein, PSCP Protein, RNF53 Protein, iris Protein, pscp Protein, brcai Protein, brcc1 Protein, rnf53 Protein, brca1 Protein, BRCA1 Protein, LOC100224649 Protein, BRCA1, DNA repair associated Protein, breast cancer 1 Protein, breast cancer 1, early onset Protein, breast cancer 1 L homeolog Protein, BRCA1 Protein, brca1 Protein, LOC755086 Protein, Brca1 Protein, brca1.L Protein

Background: Breast cancer type 1 susceptibility (BRCA1) protein is a tumor suppressor that is expressed in breast tissue cells and other tissues where it primarily functions to sense and repair DNA damage. BRCA1 is an E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. BRCA1 associates with other tumor suppressors, DNA repair proteins, and signal transducers in a large complex known as the BRCA1-associated genome surveillance complex (BASC) that functions to identify double-strand DNA breaks and either repair them or destroy cells where DNA damage cannot be repaired. BRCA1 also associates with RNA pol II and histone deacetylase complexes to modulate transcription. In addition to its role in ubiquitination, DNA damage repair and transcriptional regulation, it is known to be required for normal cell cycle progression. Defects in BRCA1 are a cause of susceptibility to familial breast-ovarian cancer type 1 (BROVCA1), a condition associated with familial predisposition to cancer of the breast and ovaries, and pancreatic cancer type 4 (PNCA4).

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus