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Histone H3.2 (biotinylated), (full length), (N-Term), (truncated) Protein

Origin: Human Host: Escherichia coli (E. coli) Recombinant Protein was determined to have ≥ 98 % purity by SDS-PAGE. PC, S
Catalog No. ABIN2669559
  • Target
    Histone H3.2
    Protein Type
    Recombinant
    Protein Characteristics
    N-Term, truncated, biotinylated, full length
    Origin
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    Human
    Source
    • 13
    • 1
    Escherichia coli (E. coli)
    Application
    Positive Control (PC), Substrate (S)
    Characteristics
    Truncated human Histone H3.2 is produced in E. coli and purified using FPLC. The purified protein is subsequently ligated to a peptide containing the histone tail with a N-terminal biotin via a native peptide bond. The full-length protein is then repurified prior to lyophilization. Protein concentration was determined using the molar extinction coefficient for Histone H3 and absorbance at 280nm. Protein was determined to have ≥ 98 % purity by SDS-PAGE. The molecular weight is 15,565 Daltons.
    Purification
    Purified using FPLC
    Purity
    Protein was determined to have ≥ 98 % purity by SDS-PAGE.
  • Application Notes
    Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/mL. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.
    Handling Advice
    Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
    Storage
    -20 °C/-80 °C
    Storage Comment
    Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
  • Target
    Histone H3.2
    Synonyms
    H3-I Protein, H3-II Protein, H3-III Protein, H3-IV Protein, H3-V Protein, H3-VI Protein, H3-VII Protein, H3-VIII Protein, H3F3A Protein, H3C2 Protein, H3C3 Protein, H3 histone, family 3C Protein, histone H3.2 Protein, Histone H3.2 Protein, H3F3C Protein, LOC504599 Protein, PVX_082615 Protein, Tsp_16072 Protein, H3C4 Protein, LOC106362349 Protein
    Background
    Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.
    Molecular Weight
    The molecular weight is 15,565 Daltons.
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