Histone H3.2 (biotinylated), (full length), (N-Term), (truncated) Protein
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
Protein was determined to have ≥ 98 % purity by SDS-PAGE.
PC, S
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- Target
- Histone H3.2
- Protein Type
- Recombinant
- Protein Characteristics
- N-Term, truncated, biotinylated, full length
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Positive Control (PC), Substrate (S)
- Characteristics
- Truncated human Histone H3.2 is produced in E. coli and purified using FPLC. The purified protein is subsequently ligated to a peptide containing the histone tail with a N-terminal biotin and trimethyl lysine 4 via a native peptide bond. The full-length protein is then repurified prior to lyophilization. Protein concentration was determined using the molar extinction coefficient for Histone H3 and absorbance at 280nm. Protein was determined to have ≥ 98 % purity by SDS-PAGE. The molecular weight is 15,607 Daltons.
- Purification
- Purified using FPLC
- Purity
- Protein was determined to have ≥ 98 % purity by SDS-PAGE.
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Histone H3.2 (biotinylated), (full length), (N-Term), (truncated) protein
Origin: Human Host: Escherichia coli (E. coli) Recombinant Protein was determined to have ≥ 98 % purity by SDS-PAGE. PC, S
Histone H3.2 protein (His tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/mL. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.
- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -20 °C/-80 °C
- Storage Comment
- Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- Target
- Histone H3.2
- Synonyms
- H3-I Protein, H3-II Protein, H3-III Protein, H3-IV Protein, H3-V Protein, H3-VI Protein, H3-VII Protein, H3-VIII Protein, H3F3A Protein, H3C2 Protein, H3C3 Protein, H3 histone, family 3C Protein, histone H3.2 Protein, Histone H3.2 Protein, H3F3C Protein, LOC504599 Protein, PVX_082615 Protein, Tsp_16072 Protein, H3C4 Protein, LOC106362349 Protein
- Background
- Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.
- Molecular Weight
- The molecular weight is 15,607 Daltons.
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