TRIM33 Protein (AA 959-1059) (His tag,DYKDDDDK Tag)

Catalog No. ABIN2669632

Product Details

Target: TRIM33 (Tripartite Motif Containing 33 (TRIM33))

Protein Type: Recombinant

Protein Characteristics: AA 959-1059

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TRIM33 protein is labelled with His tag,DYKDDDDK Tag.

Application: Binding Studies (Bind), Screening Assay (ScA)

Characteristics: The peptide corresponding to amino acids 959 - 1059 that contains the bromodomain sequences of TRIM33 (accession number NP_056990.3) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 17.7 kDa.

Application Details

Application Notes: Recombinant TRIM33 (959-1069) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Target Details

Target: TRIM33 (Tripartite Motif Containing 33 (TRIM33))

Alternative Name: TRIM33 (TRIM33 Products)

Synonyms: TRIM33 Protein, ptc7 Protein, rfg7 Protein, tf1g Protein, tif1g Protein, tifgamma Protein, MGC146665 Protein, tif1gamma Protein, ECTO Protein, PTC7 Protein, RFG7 Protein, TF1G Protein, TIF1G Protein, TIF1GAMMA Protein, TIFGAMMA Protein, ecto Protein, 8030451N04Rik Protein, AI413936 Protein, Ecto Protein, Tif1g Protein, ectodermin Protein, mKIAA1113 Protein, cb1085 Protein, id:ibd2175 Protein, mon Protein, wu:fc17f10 Protein, zgc:136680 Protein, tripartite motif containing 33 Protein, tripartite motif containing 33 L homeolog Protein, tripartite motif-containing 33 Protein, TRIM33 Protein, trim33 Protein, trim33.L Protein, Trim33 Protein

Background: Tripartite motif-containing 33 (TRIM33) protein, also known as TIF1γ, is a member of the transcriptional intermediary factor 1 (TIF1) family that control transcription and chromatin remodeling through their interaction with transcription factors. The family includes TRIM24 (TIF1α), TRIM28 (TIF1β) and TRIM33 (TIF1γ) that share a characteristic domain structure comprised of multiple histone-binding domains, an N-terminal TRIM region (containing a RING domain, B box type 1 and type 2 domains, and a coiled-coil region), and a C-terminal bromodomain and PHD finger. Bromodomains function as 'readers' of epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TRIM33 is an E3 ubiquitin protein ligase that promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. TRIM33 does not affect SMAD4 levels but rather acts as an inhibitor of the SMAD4-dependent TGFβ/BMP signaling cascade by monoubiquitinating SMAD4 and hampering its ability to form a stable complex with activated SMAD2/3. TRIM33 may also act as a transcriptional corepressor. It has also been shown to associate with SMAD2 and SMAD3 and stimulate differentiation of hematopoietic stem cells. Additionally, TRIM33 associates with TRIM24 and plays a role in the control of cell proliferation.

Molecular Weight: 17.7 kDa