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p300 Protein (AA 1041-1161) (His tag,DYKDDDDK Tag)

EP300 Origin: Human Host: Escherichia coli (E. coli) Recombinant Bind, ScA
Catalog No. ABIN2669637
  • Target See all p300 (EP300) Proteins
    p300 (EP300) (E1A Binding Protein P300 (EP300))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1041-1161
    Origin
    • 4
    • 1
    • 1
    Human
    Source
    • 3
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This p300 protein is labelled with His tag,DYKDDDDK Tag.
    Application
    Binding Studies (Bind), Screening Assay (ScA)
    Characteristics
    The peptide corresponding to amino acids 1041 - 1161 that contains the bromodomain sequences of EP300 (accession number NM_001429.3) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 19.1 kDa. It shows binding specificity for acetylated H3K36, H3K56, H4K12, H4K20 and H4K44, as well as acetylated MyoD-K99/K102.
    Top Product
    Discover our top product EP300 Protein
  • Application Notes
    Recombinant p300 (1041-1161) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
    Storage
    -80 °C
    Storage Comment
    Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
  • Target
    p300 (EP300) (E1A Binding Protein P300 (EP300))
    Alternative Name
    p300 (EP300 Products)
    Synonyms
    KAT3B Protein, RSTS2 Protein, p300 Protein, A430090G16 Protein, A730011L11 Protein, p300 HAT Protein, E1A binding protein p300 Protein, histone acetyltransferase p300 Protein, EP300 Protein, LOC446056 Protein, ep300 Protein, Ep300 Protein
    Background
    E1A binding protein p300 (EP300) or p300 regulates cellular growth and differentiation and is also important in preventing tumor growth. Its interaction with adenovirus E1A protein is thought regulate the transforming capacity of E1A. p300 is a transcriptional coactivator with histone acetyl transferase (HAT) activity. It can acetylate all four core histones and regulates transcription via chromatin remodeling. p300 also functions as an acetyltransferase for non-histone targets. Specifically, p300 acetylates 'Lys131' of ALX1 and acts as its coactivator in the presence of CREBBP. p300 is also thought to indirectly increase the transcriptional activity of p53 through acetylation of SIRT2 and subsequent attenuation of its deacetylase function. Additionally, HDAC1 acetylation by p300 leads to HDAC1 inactivation. p300 also acts as a TFAP2A-mediated transcriptional coactivator in the presence of CITED2 and as a coactivator of NEUROD1-dependent transcription of Secretin and p21. Additionally, p300 binds to phosphorylated CREB and mediates cAMP gene regulation. It also regulates terminal differentiation of intestinal epithelial cells. In the case of HIV-1 infection, p300 is recruited by the viral protein TAT and regulates TAT's transactivating activity, and may aid induction of chromatin remodeling of proviral genes. p300 contains a bromodomain that recognizes acetylated histone lysine residues and functions as a 'reader' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.
    Molecular Weight
    19.1 kDa
    Pathways
    p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size
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