KDM3A Protein (DYKDDDDK Tag)
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- Target See all KDM3A Proteins
- KDM3A (Lysine (K)-Specific Demethylase 3A (KDM3A))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Baculovirus
- Purification tag / Conjugate
- This KDM3A protein is labelled with DYKDDDDK Tag.
- Application
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- Characteristics
- Recombinant JMJD1A / KDM3A (accession number NP_001140160.1) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 151.6 kDa.
- Top Product
- Discover our top product KDM3A Protein
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Lysine (K)-Specific Demethylase 3A (KDM3A) (AA 1-1321) protein (His tag)
Crystallography grade KDM3A Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Lysine (K)-Specific Demethylase 3A (KDM3A) (AA 1-1323) protein (His tag)Crystallography grade KDM3A Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Lysine (K)-Specific Demethylase 3A (KDM3A) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)KDM3A Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Recombinant JMJD1A / KDM3A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K9me2 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 60 nM Recombinant JMJD1A / KDM3A protein, and 3.3 μM H3K9me2 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
- Restrictions
- For Research Use only
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- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -80 °C
- Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- Target
- KDM3A (Lysine (K)-Specific Demethylase 3A (KDM3A))
- Alternative Name
- JMJD1A / KDM3A (KDM3A Products)
- Background
- KDM3A (lysine (K)-specific demethylase 3A), also known as JMJD1A (Jumonji Domain Containing 1A) is a histone demethylase that preferentially demethylates mono- and dimethylated lysine 9 of histone H3, with a preference for the dimethylated residue. KDM3A has little or no activity on trimethylated lysine 9. KDM3A is involved in hormone-dependent transcriptional activation by participating in the recruitment to androgen-receptor target genes resulting in H3 lysine 9 demethylation and transcriptional activation. KDM3A is also involved in spermatogenesis where it regulates expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. KDM3A contributes to obesity resistance through its regulation of metabolic genes such as PPARα and UCP1.
- Molecular Weight
- 151.6 kDa
- Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Warburg Effect
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